Transcriptomic and methylomic analysis reveal the toxicological effect of 2,3,7,8-Tetrachlorodibenzodioxin on human embryonic stem cell

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

7 Scopus Citations
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Author(s)

  • Keng Po Lai
  • Jing Woei Li
  • Ting Fung Chan
  • Andy Chen
  • Cherie Yin Lau Lee
  • And 2 others
  • William Shu Biu Yeung
  • Chris Kong Chu Wong

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)663-673
Journal / PublicationChemosphere
Volume206
Online published10 May 2018
Publication statusPublished - Sept 2018

Abstract

Cumulating epidemiological studies demonstrated that environmental exposure to endocrine disrupting chemicals (EDCs) during the early stages of fetal development is associated with the increase in disease susceptibility in later life. The fetal developmental plasticity is considered as a protective mechanism against an undesirable prenatal environment. Dioxin is one of the environmental contaminants and is considered a diabetogenic factor. Experimental animal and human epidemiological studies have revealed that dioxin exposure was associated with insulin resistance and altered beta cell function. But the effect of dioxin exposure in early stage of fetal development is still largely unknown. In this report, we used the human embryonic stem cell (hESC) line, VAL-3, as a model, together with Methyl-CpG Binding Domain (MBD) protein-enriched genome sequencing and transcriptome sequencing (RNA-seq), in order to determine the dynamic changes of the epigenetic landscape and transcriptional dysregulation in hESC upon dioxin exposure. The bioinformatics analyses including the Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis and Ingenuity Pathway Analysis (IPA) highlighted the predisposed neural, hepatic, cardiac and metabolic toxicological effects of dioxin during the fetal development.

Research Area(s)

  • Dioxin, Embryonic stem cells, Methylome, Toxicogenomic analysis, Transcriptome

Citation Format(s)

Transcriptomic and methylomic analysis reveal the toxicological effect of 2,3,7,8-Tetrachlorodibenzodioxin on human embryonic stem cell. / Lai, Keng Po; Li, Jing Woei; Chan, Ting Fung et al.
In: Chemosphere, Vol. 206, 09.2018, p. 663-673.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review