Molecular radical cations of oligopeptides

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Ivan K. Chu
  • Christopher F. Rodriquez
  • Tai-Chu Lau
  • Alan C. Hopkinson
  • K. W. Michael Siu

Detail(s)

Original languageEnglish
Pages (from-to)3393-3397
Journal / PublicationJournal of Physical Chemistry B
Volume104
Issue number15
Publication statusPublished - 20 Apr 2000

Abstract

An unprecedented method of producing molecular radical cations of oligopeptides in the gas phase has been discovered. Electrospraying a methanolic mixture of a Cu(II)-amine complex, e.g., CuII(dien)(NO3)2 (where dien = diethylenetriamine), and an oligopeptide (M) yields the [CuII(dien)M]·2+ ion, whose collision-induced dissociation (CID) produces [CuI(dien)]+ and M·+, the molecular cation of the oligopeptide. Abundant M·+ is apparent when the oligopeptide contains both a tyrosyl and a basic residue-arginyl, lysyl, or histidyl. These structural requirements are similar to those in the metalloradical enzyme process in photosystem II. Tandem mass spectrometry of M·+ produces fragment ions that are both common to and also different from [M + H]+. The fragmentation chemistry of M·+ and of its products appear to be radical driven. © 2000 American Chemical Society.

Citation Format(s)

Molecular radical cations of oligopeptides. / Chu, Ivan K.; Rodriquez, Christopher F.; Lau, Tai-Chu et al.
In: Journal of Physical Chemistry B, Vol. 104, No. 15, 20.04.2000, p. 3393-3397.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review