An ultrafast ratiometric electrochemical biosensor based on potential-assisted hybridization for nucleic acids detection

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

5 Scopus Citations
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  • Yong Li
  • Mingyuan Zhao
  • Haixia Wang
  • Qianyi Wan
  • Chao Shi
  • Cuiping Ma

Related Research Unit(s)


Original languageEnglish
Article number339915
Journal / PublicationAnalytica Chimica Acta
Online published7 May 2022
Publication statusPublished - 8 Jun 2022


The development of rapid nucleic acid detection methods, which are promising the diagnostic standard of the infectious disease, could expand more options for disease traceability and controllability. Nucleic acid hybridization-based biosensing techniques still encounter limitations in meeting the requirements for rapid detection. Therefore, we proposed a potential-assisted ratiometric electrochemical biosensor for rapid and accurate target DNA detection. This dual-signal analysis system actuated an enzyme-free detection process. The application of an external electric field accelerated molecular dynamics, resulting in highly efficient collision chances. This hybridization process was thus improved from hours to minutes compared with passive hybridization approach. The biosensor not only had a high sensitivity with the detection limit of 12 fM, but also features a robust capability in identifying single-base mismatch. Moreover, the biosensor realized sensitive detection of target DNA in complex biological environments. Overall, this sensing strategy exhibits a promising potential for application in point-of-care testing.

Research Area(s)

  • Electrical potential–assisted hybridization, Enzyme-free detection, Nucleic acid detection, Ratiometric electrochemical biosensor