High-throughput micro capillary electrophoresis chip for bio-analytical application utilizing multi-wavelength detection

Che-Hsin Lin; Suz-Kai Hsiung; Gwo-Bin Lee

doi:10.1109/mems.2004.1290583

Author(s)

Che-Hsin Lin
Suz-Kai Hsiung
Gwo-Bin Lee

Detail(s)

Original language	English
Pages (from-to)	304-307
Journal / Publication	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Publication status	Published - 2004
Externally published	Yes

Conference

Title	17th IEEE International Conference on Micro Electro Mechanical Systems (MEMS): Maastricht MEMS 2004 Technical Digest
Place	Netherlands
City	Maastricht
Period	25 - 29 January 2004

Link(s)

DOI	DOI https://doi.org/10.1109/mems.2004.1290583 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-3042786146&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(74207b75-8b8a-4f29-9f03-c577ca757727).html

Abstract

This paper presents a novel microfluidic device for high-throughput capillary electrophoresis (CE) analysis utilizing multi-wavelength detection. In general, fluorescent dye can only be excited using a specific range of wavelength and then it will emit specific fluorescence signal with a longer wavelength. In this study, pairs of multi-mode optic fiber are embedded at the downstream of the separation channel of the CE chips for multiple-wavelength fluorescence detection. The proposed device could detect multiple samples with different kinds of fluorescence labeling in the same channel in a single run. Experimental results show that a mixture of Rhodamine B and FITC fluorescein can be detected individually while using different kinds of light source for fluorescence excitation. Sample moving speed in the microchannel and DNA fragments are also measured and detected using the proposed device.

Bibliographic Note

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Citation Format(s)

[ RIS ] [ BibTeX ]

High-throughput micro capillary electrophoresis chip for bio-analytical application utilizing multi-wavelength detection. / Lin, Che-Hsin; Hsiung, Suz-Kai; Lee, Gwo-Bin.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2004, p. 304-307.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Lin, C-H, Hsiung, S-K & Lee, G-B 2004, 'High-throughput micro capillary electrophoresis chip for bio-analytical application utilizing multi-wavelength detection', Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 304-307. https://doi.org/10.1109/mems.2004.1290583

Lin, C.-H., Hsiung, S.-K., & Lee, G.-B. (2004). High-throughput micro capillary electrophoresis chip for bio-analytical application utilizing multi-wavelength detection. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 304-307. https://doi.org/10.1109/mems.2004.1290583

Lin CH, Hsiung SK, Lee GB. High-throughput micro capillary electrophoresis chip for bio-analytical application utilizing multi-wavelength detection. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2004;304-307. doi: 10.1109/mems.2004.1290583

Lin, Che-Hsin ; Hsiung, Suz-Kai ; Lee, Gwo-Bin. / High-throughput micro capillary electrophoresis chip for bio-analytical application utilizing multi-wavelength detection. In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2004 ; pp. 304-307.