B2 Bradykinin Receptors in NG108-15 Cells: cDNA Cloning and Functional Expression

Shigeru Yokoyama; Yasuhiro Kimura; Megumi Taketo; Joel A. Black; Bruce R. Ransom; Haruhiro Higashida

doi:10.1006/bbrc.1994.1495

Author(s)

Shigeru Yokoyama
Yasuhiro Kimura
Megumi Taketo
Joel A. Black
Haruhiro Higashida

Detail(s)

Original language	English
Pages (from-to)	634-641
Journal / Publication	Biochemical and Biophysical Research Communications
Volume	200
Issue number	1
Publication status	Published - 15 Apr 1994
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1006/bbrc.1994.1495 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0028358681&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(7331c29b-1e08-4792-b0d5-e41a3fe1dc72).html

Abstract

Two distinct cDNAs encoding bradykinin receptors (BKRs) were cloned from NG108-15 neuroblastoma-glioma hybrid cells. One was identical with rat uterus B2 BKR, whereas the other one (mBKR) had 91 % amino acid homology to the rat B2 BKR and 82 % homology to human B2 BKR. Southern blot analysis and genomic DNA cloning revealed that mBKR is derived from the mouse genome. The mBKR, expressed in Xenopus oocytes and COS-7 cells, produced functional BKRs that exhibited the properties of smooth muscle type B2 BKR. These results suggest that both the rat and mouse B2 BKRs of the smooth muscle type are expressed in NG108-15 cells. © 1994 Academic Press, Inc.

Research Area(s)

SDG 3 - Good Health and Well-being

Citation Format(s)

[ RIS ] [ BibTeX ]

B2 Bradykinin Receptors in NG108-15 Cells: cDNA Cloning and Functional Expression. / Yokoyama, Shigeru; Kimura, Yasuhiro; Taketo, Megumi et al.
In: Biochemical and Biophysical Research Communications, Vol. 200, No. 1, 15.04.1994, p. 634-641.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Yokoyama, S, Kimura, Y, Taketo, M, Black, JA, Ransom, BR & Higashida, H 1994, 'B2 Bradykinin Receptors in NG108-15 Cells: cDNA Cloning and Functional Expression', Biochemical and Biophysical Research Communications, vol. 200, no. 1, pp. 634-641. https://doi.org/10.1006/bbrc.1994.1495

Yokoyama, S., Kimura, Y., Taketo, M., Black, J. A., Ransom, B. R., & Higashida, H. (1994). B2 Bradykinin Receptors in NG108-15 Cells: cDNA Cloning and Functional Expression. Biochemical and Biophysical Research Communications, 200(1), 634-641. https://doi.org/10.1006/bbrc.1994.1495

Yokoyama S, Kimura Y, Taketo M, Black JA, Ransom BR, Higashida H. B2 Bradykinin Receptors in NG108-15 Cells: cDNA Cloning and Functional Expression. Biochemical and Biophysical Research Communications. 1994 Apr 15;200(1):634-641. doi: 10.1006/bbrc.1994.1495

Yokoyama, Shigeru ; Kimura, Yasuhiro ; Taketo, Megumi et al. / B2 Bradykinin Receptors in NG108-15 Cells : cDNA Cloning and Functional Expression. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 200, No. 1. pp. 634-641.

B2 Bradykinin Receptors in NG108-15 Cells : cDNA Cloning and Functional Expression