Membrane-disruptive engineered peptide amphiphiles restrain the proliferation of penicillins and cephalosporins resistant Vibrio alginolyticus and Vibrio parahaemolyticus in instant jellyfish

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

1 Scopus Citations
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Author(s)

  • Ping Zeng
  • Qipeng Cheng
  • Jiangtao Xu
  • Wei Gao
  • Kwok-Yin Wong
  • Kin Fai Chan
  • Lanhua Yi

Detail(s)

Original languageEnglish
Article number108827
Journal / PublicationFood Control
Volume135
Online published12 Jan 2022
Publication statusPublished - May 2022

Abstract

Over 1 billion people rely on seafood as their primary source of protein and Vibrio spp. is the most important pathogen of foodborne disease outbreak related to consumption of seafood. Moreover, the rise of drug-resistant Vibrio strains further increases the difficulty of clinical treatment. However, there is no food preservative available to combat drug-resistant Vibrio spp. in seafood. In this study, we designed and synthesized three novel antibacterial peptides (ABPs) derived from zp3 (GIIAGIIIKIKK-NH2). One of them, 3K5K7G (GIKAKIGIKIKK-NH2), had bioactivity against all tested penicillins and cephalosporins resistant Vibrio with minimal inhibitory concentration (MIC) values at 2–16 μM, performing better than its template zp3. 3K5K7G demonstrated favourable antibacterial capability in instant jellyfish, killing more than 99% Vibrio alginolyticus cells after 64 μM treatment for 4 h. Peptide 3K5K7G could disrupt the cell membrane, resulting in rapid membrane potential dissipation. Next, this molecule could penetrate inside cytoplasm and further bind with DNA. Importantly, 3K5K7G showed low cytotoxicity to mammalian cells (95% Vero cells survived in the presence of 160 μM 3K5K7G). Collectively, 3K5K7G has the potential to be developed into a promising food-grade antibacterial additive, especially against those resistant Vibrio spp. in seafood.

Research Area(s)

  • Antibacterial peptide, Drug-resistant strain, Membrane damage, Seafood, Vibrio

Citation Format(s)

Membrane-disruptive engineered peptide amphiphiles restrain the proliferation of penicillins and cephalosporins resistant Vibrio alginolyticus and Vibrio parahaemolyticus in instant jellyfish. / Zeng, Ping; Cheng, Qipeng; Xu, Jiangtao; Xu, Qi; Xu, Yating; Gao, Wei; Wong, Kwok-Yin; Chan, Kin Fai; Chen, Sheng; Yi, Lanhua.

In: Food Control, Vol. 135, 108827, 05.2022.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review