Syntheses of ruthenium(II) quinonediimine complexes of cyclam and characterization of their DNA-binding activities and cytotoxicity

Hing-Leung Chan; Heng-Qian Liu; Bling-Chiau Tzeng; Yuan-Shyane You; Shie-Ming Peng; Mengsu Yang; Chi-Ming Che

doi:10.1021/ic0112802

Author(s)

Hing-Leung Chan
Heng-Qian Liu
Bling-Chiau Tzeng
Yuan-Shyane You
Shie-Ming Peng
Chi-Ming Che

Detail(s)

Original language	English
Pages (from-to)	3161-3171
Journal / Publication	Inorganic Chemistry
Volume	41
Issue number	12
Publication status	Published - 17 Jun 2002

Link(s)

DOI	DOI https://doi.org/10.1021/ic0112802 Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0037124236&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(69740c4b-31f6-4973-9fe3-13d887943fd2).html

Abstract

The synthesis and characterization of ruthenium(II) complexes, [Ru(cyclam)(bqdi)]·ZnCl4 (1·ZnCl₄; cyclam = 1,4,8,11-tetraazacyclotetradecane, bqdi = o-benzoquinonediimine), [Ru(cyclam)(nqdi)l·(ClO₄)₂ (2·(ClO₄)₂ nqdi = 2,3- naphthoquinonediimine), and [Ru(cyclam)(phi)]·(ClO₄)₂ (3·(ClO₄)₂); phi = 9,10- phenanthroquinonediimine), are described. The DNA binding properties and biological activity of the Ru(II) complexes were studied by various biophysical and cytological techniques. As expected, only 3 showed significant binding with DNA. The thermodynamic profile of the binding of 3 and DNA was constructed by analyzing the experimental data of absorption titration and UV melting studies with the McGhee equation, van't Hoff's equation, and the Gibbs-Helmholtz equation. Compound 3 binds double-stranded DNA with a binding constant of 5.0 × 10⁴ M^-1 at 20 °C, and the binding mode of the complex to DNA was proved to be intercalative. Cytotoxicity and induced type of cell death of 1-3 were also investigated. Basically, metal complexes with ligands of molecular shape closely related to the structure of DNA are more likely to bind DNA and possess higher toxicity.

Citation Format(s)

[ RIS ] [ BibTeX ]

Syntheses of ruthenium(II) quinonediimine complexes of cyclam and characterization of their DNA-binding activities and cytotoxicity. / Chan, Hing-Leung; Liu, Heng-Qian; Tzeng, Bling-Chiau et al.
In: Inorganic Chemistry, Vol. 41, No. 12, 17.06.2002, p. 3161-3171.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Chan, H-L, Liu, H-Q, Tzeng, B-C, You, Y-S, Peng, S-M, Yang, M & Che, C-M 2002, 'Syntheses of ruthenium(II) quinonediimine complexes of cyclam and characterization of their DNA-binding activities and cytotoxicity', Inorganic Chemistry, vol. 41, no. 12, pp. 3161-3171. https://doi.org/10.1021/ic0112802

Chan, H.-L., Liu, H.-Q., Tzeng, B.-C., You, Y.-S., Peng, S.-M., Yang, M., & Che, C.-M. (2002). Syntheses of ruthenium(II) quinonediimine complexes of cyclam and characterization of their DNA-binding activities and cytotoxicity. Inorganic Chemistry, 41(12), 3161-3171. https://doi.org/10.1021/ic0112802

Chan HL, Liu HQ, Tzeng BC, You YS, Peng SM, Yang M et al. Syntheses of ruthenium(II) quinonediimine complexes of cyclam and characterization of their DNA-binding activities and cytotoxicity. Inorganic Chemistry. 2002 Jun 17;41(12):3161-3171. doi: 10.1021/ic0112802

Chan, Hing-Leung ; Liu, Heng-Qian ; Tzeng, Bling-Chiau et al. / Syntheses of ruthenium(II) quinonediimine complexes of cyclam and characterization of their DNA-binding activities and cytotoxicity. In: Inorganic Chemistry. 2002 ; Vol. 41, No. 12. pp. 3161-3171.