Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: A real time scanning tunnelling microscopy study

R. Mukhopadhyay; K. K. Lo; L. L. Wong; H. A O Hill

doi:10.1111/j.1365-2818.2004.01246.x

Author(s)

R. Mukhopadhyay
K. K. Lo
L. L. Wong
H. A O Hill

Detail(s)

Original language	English
Pages (from-to)	6-10
Journal / Publication	Journal of Microscopy
Volume	213
Issue number	1
Publication status	Published - Jan 2004

Link(s)

DOI	DOI https://doi.org/10.1111/j.1365-2818.2004.01246.x Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0742304124&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(64fb8bf6-54ba-4785-aabf-21326cf1c486).html

Abstract

A scanning tunnelling microscopy study of adsorption of wild-type Pseudomonas putida putidaredoxin at a gold (111)-buffer interface has been made in real time. Reversible adsorption has been observed reflecting weak interaction of the wild-type protein with a gold (111) electrode. A genetically engineered mutant, C73S-D58C. which contains a surface thiol, has been used for 'immobilization' and 'orientated adsorption' on the gold surface. The implication of such orientated immobilization in development of a bio-electrode surface has been predicted.

Research Area(s)

Adsorption, Gold (111), Interface, Protein immobilization, Putidaredoxin, Real-time study, Scanning tunnelling microscopy, STM

Citation Format(s)

[ RIS ] [ BibTeX ]

Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: A real time scanning tunnelling microscopy study. / Mukhopadhyay, R.; Lo, K. K.; Wong, L. L. et al.
In: Journal of Microscopy, Vol. 213, No. 1, 01.2004, p. 6-10.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Mukhopadhyay, R, Lo, KK, Wong, LL & Hill, HAO 2004, 'Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: A real time scanning tunnelling microscopy study', Journal of Microscopy, vol. 213, no. 1, pp. 6-10. https://doi.org/10.1111/j.1365-2818.2004.01246.x

Mukhopadhyay, R., Lo, K. K., Wong, L. L., & Hill, H. A. O. (2004). Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: A real time scanning tunnelling microscopy study. Journal of Microscopy, 213(1), 6-10. https://doi.org/10.1111/j.1365-2818.2004.01246.x

Mukhopadhyay R, Lo KK, Wong LL, Hill HAO. Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: A real time scanning tunnelling microscopy study. Journal of Microscopy. 2004 Jan;213(1):6-10. doi: 10.1111/j.1365-2818.2004.01246.x

Mukhopadhyay, R. ; Lo, K. K. ; Wong, L. L. et al. / Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface : A real time scanning tunnelling microscopy study. In: Journal of Microscopy. 2004 ; Vol. 213, No. 1. pp. 6-10.

Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface : A real time scanning tunnelling microscopy study