Application of CRISPR/Cas9-Based Genome Editing in Studying the Mechanism of Pandrug Resistance in Klebsiella pneumoniae
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
Detail(s)
| Original language | English |
|---|---|
| Article number | e00113-19 |
| Journal / Publication | Antimicrobial Agents and Chemotherapy |
| Volume | 63 |
| Issue number | 7 |
| Online published | 24 Jun 2019 |
| Publication status | Published - Jul 2019 |
| Externally published | Yes |
Link(s)
Abstract
In this study, a CRISPR/Cas9-mediated genome editing method was used to study the functions of the mgrB, tetA, and ramR genes in mediating colistin and tigecycline resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP). Inactivation of the tetA or ramR gene or the mgrB gene by CRISPR/Cas9 affected bacterial susceptibility to tigecycline or colistin, respectively. This study proved that the CRISPR/Cas9-based genome editing method could be effectively applied to K. pneumoniae and should be further utilized for genetic characterization.
Research Area(s)
- CRISPR/Cas9, Drug resistance mechanisms, Genome editing, Klebsiella pneumoniae, Pandrug resistance
Citation Format(s)
Application of CRISPR/Cas9-Based Genome Editing in Studying the Mechanism of Pandrug Resistance in Klebsiella pneumoniae. / Sun, Qiaoling; Wang, Yu; Dong, Ning; Shen, Lingwei; Zhou, Hongwei; Hu, Yanyan; Gu, Danxia; Chen, Sheng; Zhang, Rong; Ji, Quanjiang.
In: Antimicrobial Agents and Chemotherapy, Vol. 63, No. 7, e00113-19, 07.2019.Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
