Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
| Original language | English |
|---|---|
| Pages (from-to) | 5293-5297 |
| Journal / Publication | Angewandte Chemie - International Edition |
| Volume | 59 |
| Issue number | 13 |
| Online published | 23 Jan 2020 |
| Publication status | Published - 23 Mar 2020 |
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Abstract
G-quadruplex (G4) structures are of general importance in chemistry and biology, such as in biosensing, gene regulation, and cancers. Although a large repertoire of G4-binding tools has been developed, no aptamer has been developed to interact with G4. Moreover, the G4 selectivity of current toolkits is very limited. Herein, we report the first L-RNA aptamer that targets a D-RNA G-quadruplex (rG4). Using TERRA rG4 as an example, our results reveal that this l-RNA aptamer, Ap3-7, folds into a unique secondary structure, exhibits high G4 selectivity and effectively interferes with TERRA-rG4–RHAU53 binding. Our approach and findings open a new door in further developing G4-specific tools for diverse applications.
Research Area(s)
- aptamers, G-quadruplexes, nucleic acids, RNA, RNA structures
Citation Format(s)
Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer. / Chan, Chun-Yin; Kwok, Chun Kit.
In: Angewandte Chemie - International Edition, Vol. 59, No. 13, 23.03.2020, p. 5293-5297.Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
