Transcriptomic analysis reveals transgenerational effect of hypoxia on the neural control of testicular functions

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

1 Scopus Citations
View graph of relations

Author(s)

  • Jing Woei Li
  • Simon Yuan Wang
  • Ting Fung Chan
  • Wing Yee Lui
  • Rudolf Shiu-Sun Wu

Detail(s)

Original languageEnglish
Pages (from-to)41-48
Journal / PublicationAquatic Toxicology
Volume195
Online published16 Dec 2017
Publication statusPublished - Feb 2018

Abstract

There are over 400 hypoxic zones in the ocean worldwide. Both laboratory and field studies have shown that hypoxia causes endocrine disruption and reproductive impairments in vertebrates. More importantly, our recent study discovered that parental (F0) hypoxia exposure resulted in the transgenerational impairment of sperm quality in the F2 generation through the epigenetic regulation of germ cells. In the present study, we aim to test the hypothesis that the brain, as the major regulator of the brain-pituitary-gonad (BPG) axis, is also involved in the observed transgenerational effect. Using comparative transcriptomic analysis on brain tissues of marine medaka Oryzias melastigma, 45 common differentially expressed genes caused by parental hypoxia exposure were found in the hypoxic group of the F0 and F2 generations, and the transgenerational groups of the F2 generation. The bioinformatic analysis on this deregulated gene cluster further highlighted the possible involvement of the brain in the transgenerational effect of hypoxia on testicular structure, including abnormal morphologies of the epididymis and the seminal vesicle, and degeneration of the seminiferous tubule. This finding is concordant to the result of hematoxylin and eosin staining, which showed the reduction of testicular lobular diameter in the F0 and F2 generations. Our study demonstrated for the first time the involvement of the brain in the transgenerational effect of hypoxia.

Research Area(s)

  • Brain, Hypoxia, Medaka, Transcriptome, Transgeneration

Citation Format(s)

Transcriptomic analysis reveals transgenerational effect of hypoxia on the neural control of testicular functions. / Lai, Keng Po; Li, Jing Woei; Wang, Simon Yuan; Wan, Miles Teng; Chan, Ting Fung; Lui, Wing Yee; Au, Doris Wai-Ting; Wu, Rudolf Shiu-Sun; Kong, Richard Yuen-Chong.

In: Aquatic Toxicology, Vol. 195, 02.2018, p. 41-48.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review