Video-rate functional photoacoustic microscopy at depths

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Lidai Wang
  • Konstantin Maslov
  • Wenxin Xing
  • Alejandro Garcia-Uribe
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Article number106007
Journal / PublicationJournal of Biomedical Optics
Volume17
Issue number10
Publication statusPublished - Oct 2012
Externally publishedYes

Abstract

We report the development of functional photoacoustic microscopy capable of video-rate high-resolution in vivo imaging in deep tissue. A lightweight photoacoustic probe is made of a single-element broadband ultrasound transducer, a compact photoacoustic beam combiner, and a bright-field light delivery system. Focused broadband ultrasound detection provides a 44-μm lateral resolution and a 28-μm axial resolution based on the envelope (a 15-μm axial resolution based on the raw RF signal). Due to the efficient bright-field light delivery, the system can image as deep as 4.8 mm in vivo using low excitation pulse energy (28 μJ per pulse, 0.35 mJ/Mcm2 on the skin surface). The photoacoustic probe is mounted on a fast-scanning voice-coil scanner to acquire 40 two-dimensional (2-D) B-scan images per second over a 9-mm range. High-resolution anatomical imaging is demonstrated in the mouse ear and brain. Via fast dual-wavelength switching, oxygen dynamics of mouse cardio-vasculature is imaged in realtime as well.© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).

Research Area(s)

  • Cardio-vascular hemodynamics, Fast scanning, Oxygen dynamics, Photoacoustic imaging, Photoacoustic microscopy, Photoacoustic tomography, Voice coil

Citation Format(s)

Video-rate functional photoacoustic microscopy at depths. / Wang, Lidai; Maslov, Konstantin; Xing, Wenxin et al.
In: Journal of Biomedical Optics, Vol. 17, No. 10, 106007, 10.2012.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review