A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

Chi-Kin Koo; Ka-Leung Wong; Cornelia Wing-Yin Man; Yun-Wah Lam; Leo King-Yan So; Hoi-Lam Tam; Sai-Wah Tsao; Kok-Wai Cheah; Kai-Chung Lau; Yang-Yi Yang; Jin-Can Chen; Michael Hon-Wah Lam

doi:10.1021/ic801261h

Author(s)

Chi-Kin Koo
Ka-Leung Wong
Cornelia Wing-Yin Man
Yun-Wah Lam
Leo King-Yan So
Hoi-Lam Tam
Sai-Wah Tsao
Kok-Wai Cheah
Yang-Yi Yang
Jin-Can Chen
Michael Hon-Wah Lam

Detail(s)

Original language	English
Pages (from-to)	872-878
Journal / Publication	Inorganic Chemistry
Volume	48
Issue number	3
Publication status	Published - 2 Feb 2009

Link(s)

DOI	DOI https://doi.org/10.1021/ic801261h Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-60849111428&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(43e5bb43-0a5f-4673-a17f-b1734830ed5a).html

Abstract

The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C _phenyl, N_pyridyl, and N_pyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.

Citation Format(s)

[ RIS ] [ BibTeX ]

A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging. / Koo, Chi-Kin; Wong, Ka-Leung; Man, Cornelia Wing-Yin et al.

In: Inorganic Chemistry, Vol. 48, No. 3, 02.02.2009, p. 872-878.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Koo, C-K, Wong, K-L, Man, CW-Y, Lam, Y-W, So, LK-Y, Tam, H-L, Tsao, S-W, Cheah, K-W, Lau, K-C, Yang, Y-Y, Chen, J-C & Lam, MH-W 2009, 'A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging', Inorganic Chemistry, vol. 48, no. 3, pp. 872-878. https://doi.org/10.1021/ic801261h

Koo, C-K., Wong, K-L., Man, C. W-Y., Lam, Y-W., So, L. K-Y., Tam, H-L., Tsao, S-W., Cheah, K-W., Lau, K-C., Yang, Y-Y., Chen, J-C., & Lam, M. H-W. (2009). A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging. Inorganic Chemistry, 48(3), 872-878. https://doi.org/10.1021/ic801261h

Koo C-K, Wong K-L, Man CW-Y, Lam Y-W, So LK-Y, Tam H-L et al. A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging. Inorganic Chemistry. 2009 Feb 2;48(3):872-878. doi: 10.1021/ic801261h

Koo, Chi-Kin ; Wong, Ka-Leung ; Man, Cornelia Wing-Yin et al. / A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging. In: Inorganic Chemistry. 2009 ; Vol. 48, No. 3. pp. 872-878.