Urinary arsenic methylation and porphyrin profile of C57Bl/6J mice chronically exposed to sodium arsenate

Manonmanii Krishnamohan; Hui-Jie Wu; Shu-Huei Huang; Robyn Maddelena; Paul K.S. Lam; Michael R. Moore; Jack C. Ng

doi:10.1016/j.scitotenv.2006.08.052

Author(s)

Manonmanii Krishnamohan
Hui-Jie Wu
Shu-Huei Huang
Robyn Maddelena
Paul K.S. Lam
And 2 others

Michael R. Moore
Jack C. Ng

Detail(s)

Original language	English
Pages (from-to)	235-243
Journal / Publication	Science of the Total Environment
Volume	379
Issue number	2-3
Publication status	Published - 1 Jul 2007

Link(s)

DOI	DOI https://doi.org/10.1016/j.scitotenv.2006.08.052 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-34248643129&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(402f575a-db44-4e63-a5ba-129e8f2d97e3).html

Abstract

Arsenic interferes with the function of enzymes responsible for haem biosynthesis leading to alteration in the porphyrin profile. In this study, young female C57Bl/6J mice were given drinking water containing 0, 100, 250 and 500 μg As^V/L as sodium arsenate ad libitum for 24 months. 24 h pooled urine samples were collected bimonthly for urinary arsenic methylation and porphyrin analyses by HPLC-ICP-MS and HPLC respectively. The levels of total arsenic were significantly dose related except for the 2nd month interval. No significant differences in the urinary arsenic methylation pattern between control and test groups were observed. Coproporphyrin I (Copro I) showed a significant dose-response relationship after 12, 14 and 20 months of exposure. Significant differences in the levels of coproporphyrin III (Copro III) were observed in the 8th month in 250 and 500 μg/L treatment groups and the dose-response pattern was maintained after 10 and 12 months. Our results suggest that urinary arsenic is a useful biomarker for internal dose, and that urinary coproporphyrin can be used as an early warning biomarker of effects before the onset of cancer. Crown Copyright © 2006.

Research Area(s)

Arsenic, Biomarker, Chronic exposure, Methylation, Mice, Porphyrin

Citation Format(s)

[ RIS ] [ BibTeX ]

Urinary arsenic methylation and porphyrin profile of C57Bl/6J mice chronically exposed to sodium arsenate. / Krishnamohan, Manonmanii; Wu, Hui-Jie; Huang, Shu-Huei; Maddelena, Robyn; Lam, Paul K.S.; Moore, Michael R.; Ng, Jack C.

In: Science of the Total Environment, Vol. 379, No. 2-3, 01.07.2007, p. 235-243.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Krishnamohan, M, Wu, H-J, Huang, S-H, Maddelena, R, Lam, PKS, Moore, MR & Ng, JC 2007, 'Urinary arsenic methylation and porphyrin profile of C57Bl/6J mice chronically exposed to sodium arsenate', Science of the Total Environment, vol. 379, no. 2-3, pp. 235-243. https://doi.org/10.1016/j.scitotenv.2006.08.052

Krishnamohan, M., Wu, H-J., Huang, S-H., Maddelena, R., Lam, P. K. S., Moore, M. R., & Ng, J. C. (2007). Urinary arsenic methylation and porphyrin profile of C57Bl/6J mice chronically exposed to sodium arsenate. Science of the Total Environment, 379(2-3), 235-243. https://doi.org/10.1016/j.scitotenv.2006.08.052

Krishnamohan M, Wu H-J, Huang S-H, Maddelena R, Lam PKS, Moore MR et al. Urinary arsenic methylation and porphyrin profile of C57Bl/6J mice chronically exposed to sodium arsenate. Science of the Total Environment. 2007 Jul 1;379(2-3):235-243. https://doi.org/10.1016/j.scitotenv.2006.08.052

Krishnamohan, Manonmanii ; Wu, Hui-Jie ; Huang, Shu-Huei ; Maddelena, Robyn ; Lam, Paul K.S. ; Moore, Michael R. ; Ng, Jack C. / Urinary arsenic methylation and porphyrin profile of C57Bl/6J mice chronically exposed to sodium arsenate. In: Science of the Total Environment. 2007 ; Vol. 379, No. 2-3. pp. 235-243.