Andrographolide isolated from Andrographis paniculata induces cell cycle arrest and mitochondrial-mediated apoptosis in human leukemic HL-60 cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Hon-Yeung Cheung
  • Jieliang Li
  • Chui-Shan Cheung
  • Wai-Ping Lai
  • Wang-Fun Fong
  • And 1 others
  • Fo-Man Leung

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)1106-1111
Journal / PublicationPlanta Medica
Volume71
Issue number12
Publication statusPublished - Dec 2005

Abstract

The in vitro cytotoxicities of the ethanol extract of Andrographis paniculata (APE) and its main diterpenoid components were evaluated in various cancer cells. APE was found to be significantly growth inhibitory to human acute myeloid leukemic HL-60 cells with an IC50 value of 14.01 μg/mL after 24 h of treatment. Among the three main diterpenoids in A paniculata, andrographolide exhibited the highest degree of cytotoxicity followed by deoxyandrographolide while neoandrographolide was the least effective. Laser confocal microscopy and gel electrophoresis studies revealed chromosomal DNA fragmentations suggesting the occurrence of apoptosis. An increase of G 0/G1 phase cells from 51.88% to 78.69% was noted after andrographolide treatment for 36 h. The G0/G1 phase arrest and apoptosis were associated with disappearance of mitochondrial cytochrome c and increased expression of Bax but decreased expression of Bcl-2 proteins in the inhibited cells. Although the order of all these events has not been determined, it is concluded that APE and andrographolide induce cell cycle arrest and affect an intrinsic mitochondria-dependent pathway of apoptosis by regulating the expression of some pro-apoptotic markers in HL-60 cells. © Georg Thieme Verlag KG Stuttgart.

Research Area(s)

  • Acanthaceae, Andrographis paniculata, Andrographolide, Apoptosis, Bax, Bcl-2 family, Cell cycle, Cytochrome c

Citation Format(s)

Andrographolide isolated from Andrographis paniculata induces cell cycle arrest and mitochondrial-mediated apoptosis in human leukemic HL-60 cells. / Cheung, Hon-Yeung; Li, Jieliang; Cheung, Chui-Shan et al.
In: Planta Medica, Vol. 71, No. 12, 12.2005, p. 1106-1111.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review