Rapid and sustained restoration of astrocytic functions by ketamine in depression model mice

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

View graph of relations

Author(s)

  • Xiaoyan Ma
  • Shuo Yang
  • Zhaoxiang Zhang
  • Luping Liu
  • Wenli Shi
  • Shupeng Li
  • Xiang Cai
  • Qiang Zhou

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)89-94
Number of pages6
Journal / PublicationBiochemical and Biophysical Research Communications
Volume616
Online published14 Apr 2022
Publication statusPublished - 6 Aug 2022

Abstract

Molecules with fast-acting antidepressant effects have potentials to become new antidepressants. Here we report the fast-acting (1hr) antidepressant effects of ketamine (10 mg/kg, i.p.) in chronic adreno-cortico-tropic-hormone (ACTH)-induced and chronic unpredictable mild stress (CUMS)-induced depression mouse models. These behavioral anti-depressant effects are associated with normalized expression of glutamate transporter-1(GLT-1), glial fibrillary acidic protein (GFAP), brain-derived neurotrophic factor (BDNF) and eukaryotic elongation factor 2 phosphorylation (p-eEF2) in the prelimbic prefrontal cortex (PrL-PFC). Excitatory neurons in PrL also showed reduced ambient glutamate responses to synaptic stimulation, and reduced ambient NMDA receptor responses after ketamine injection. Interestingly, ketamine induced biochemical and electrophysiological changes still occurred with GLT-1 knockdown in PrL, suggesting that elevated GLT-1 level is not required for ketamine to exert its antidepressant effect. At the same time, ketamine did not elevate GLT-1 level in the isolated astrocytes, suggesting distinct contributions from neurons and astrocytes to ketamine-induced changes.

Research Area(s)

  • GLT-1, Rapid reversal, Ketamine, Anti-depressant, Astrocyte, Neuron

Citation Format(s)

Rapid and sustained restoration of astrocytic functions by ketamine in depression model mice. / Ma, Xiaoyan; Yang, Shuo ; Zhang, Zhaoxiang; Liu, Luping; Shi, Wenli; Yang, Sungchil; Li, Shupeng; Cai, Xiang; Zhou, Qiang.

In: Biochemical and Biophysical Research Communications, Vol. 616, 06.08.2022, p. 89-94.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review