COMRADES determines in vivo RNA structures and interactions

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Omer Ziv
  • Marta M. Gabryelska
  • Aaron T. L. Lun
  • Luca F. R. Gebert
  • Jessica Sheu-Gruttadauria
  • Luke W. Meredith
  • Zhong-Yu Liu
  • Cheng-Feng Qin
  • Ian J. MacRae
  • Ian Goodfellow
  • John C. Marioni
  • Grzegorz Kudla
  • Eric A. Miska

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Detail(s)

Original languageEnglish
Pages (from-to)785-788
Journal / PublicationNature Methods
Volume15
Issue number10
Online published10 Sep 2018
Publication statusPublished - Oct 2018

Abstract

The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs.

Citation Format(s)

COMRADES determines in vivo RNA structures and interactions. / Ziv, Omer; Gabryelska, Marta M.; Lun, Aaron T. L.; Gebert, Luca F. R.; Sheu-Gruttadauria, Jessica; Meredith, Luke W.; Liu, Zhong-Yu; Kwok, Chun Kit; Qin, Cheng-Feng; MacRae, Ian J.; Goodfellow, Ian; Marioni, John C.; Kudla, Grzegorz; Miska, Eric A.

In: Nature Methods, Vol. 15, No. 10, 10.2018, p. 785-788.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review