Hypoxia induces the activation of human hepatic stellate cells LX-2 through TGF-β signaling pathway

Yue-Feng Shi; Chi-Chun Fong; Qi Zhang; Pik-Yuen Cheung; Chi-Hung Tzang; Rudolf S.S. Wu; Mengsu Yang

doi:10.1016/j.febslet.2006.12.010

Author(s)

Yue-Feng Shi
Chi-Chun Fong
Qi Zhang
Pik-Yuen Cheung
Chi-Hung Tzang
Rudolf S.S. Wu

Detail(s)

Original language	English
Pages (from-to)	203-210
Journal / Publication	FEBS Letters
Volume	581
Issue number	2
Publication status	Published - 23 Jan 2007

Link(s)

DOI	DOI https://doi.org/10.1016/j.febslet.2006.12.010 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-33846209631&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(1d3bbbb6-93e2-4070-8dae-e697de18b72b).html

Abstract

Hypoxia is a common environmental stress factor and is also associated with various physiological and pathological conditions such as fibrogenesis. The activation of hepatic stellate cells (HSCs) is the key event in the liver fibrogenesis. In this study, the behavior of human HSCs LX-2 in low oxygen tension (1% O₂) was analyzed. Upon hypoxia, the expression of HIF-1α and VEGF gene was induced. The result of Western blotting showed that the expression of α-SMA was increased by hypoxic stimulation. Furthermore, the expression of MMP-2 and TIMP-1 genes was increased. Hypoxia also elevated the protein expression of the collagen type I in LX-2 cells. The analysis of TGF-β/Smad signaling pathway showed that hypoxia potentiated the expression of TGF-β1 and the phosphorylation status of Smad2. Gene expression profiles of LX-2 cells induced by hypoxia were obtained by using cDNA microarray technique. © 2006 Federation of European Biochemical Societies.

Research Area(s)

Fibrogenesis, Hepatic stellate cells, Hypoxia

Citation Format(s)

[ RIS ] [ BibTeX ]

Hypoxia induces the activation of human hepatic stellate cells LX-2 through TGF-β signaling pathway. / Shi, Yue-Feng; Fong, Chi-Chun; Zhang, Qi et al.
In: FEBS Letters, Vol. 581, No. 2, 23.01.2007, p. 203-210.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Shi, Y-F, Fong, C-C, Zhang, Q, Cheung, P-Y, Tzang, C-H, Wu, RSS & Yang, M 2007, 'Hypoxia induces the activation of human hepatic stellate cells LX-2 through TGF-β signaling pathway', FEBS Letters, vol. 581, no. 2, pp. 203-210. https://doi.org/10.1016/j.febslet.2006.12.010

Shi, Y.-F., Fong, C.-C., Zhang, Q., Cheung, P.-Y., Tzang, C.-H., Wu, R. S. S., & Yang, M. (2007). Hypoxia induces the activation of human hepatic stellate cells LX-2 through TGF-β signaling pathway. FEBS Letters, 581(2), 203-210. https://doi.org/10.1016/j.febslet.2006.12.010

Shi YF, Fong CC, Zhang Q, Cheung PY, Tzang CH, Wu RSS et al. Hypoxia induces the activation of human hepatic stellate cells LX-2 through TGF-β signaling pathway. FEBS Letters. 2007 Jan 23;581(2):203-210. doi: 10.1016/j.febslet.2006.12.010

Shi, Yue-Feng ; Fong, Chi-Chun ; Zhang, Qi et al. / Hypoxia induces the activation of human hepatic stellate cells LX-2 through TGF-β signaling pathway. In: FEBS Letters. 2007 ; Vol. 581, No. 2. pp. 203-210.