All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

4 Scopus Citations
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Author(s)

  • Amelie C. F. Bergs
  • Jana F. Liewald
  • Silvia Rodriguez-Rozada
  • Christin Wirt
  • Artur Bessel
  • Nadja Zeitzschel
  • Hilal Durmaz
  • Adrianna Nozownik
  • Holger Dill
  • Maëlle Jospin
  • Johannes Vierock
  • Cornelia I. Bargmann
  • Peter Hegemann
  • J. Simon Wiegert
  • Alexander Gottschalk

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Detail(s)

Original languageEnglish
Article number1939
Journal / PublicationNature Communications
Volume14
Online published6 Apr 2023
Publication statusPublished - 2023

Link(s)

Abstract

Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits can quickly adapt, allowing perturbation, but not true control. Hence, we established an optogenetic voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional optogenetic actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping the cell to distinct potentials. We established the OVC in muscles and neurons of Caenorhabditis elegans, and transferred it to rat hippocampal neurons in slice culture. Fluorescence signals were calibrated to electrically measured potentials, and wavelengths to currents, enabling to determine optical I/V-relationships. The OVC reports on homeostatically altered cellular physiology in mutants and on Ca2+-channel properties, and can dynamically clamp spiking in C. elegans. Combining non-invasive imaging with control capabilities of electrophysiology, the OVC facilitates high-throughput, contact-less electrophysiology in individual cells and paves the way for true optogenetic control in behaving animals. © The Author(s) 2023.

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Citation Format(s)

All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals. / Bergs, Amelie C. F.; Liewald, Jana F.; Rodriguez-Rozada, Silvia et al.
In: Nature Communications, Vol. 14, 1939, 2023.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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