MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS
Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45) › 32_Refereed conference paper (with ISBN/ISSN) › peer-review
Author(s)
Detail(s)
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 280-282 |
| Volume | 1 |
| ISBN (Electronic) | 9780979806490 |
| ISBN (Print) | 9781510834163 |
| Publication status | Published - Oct 2016 |
| Externally published | Yes |
Conference
| Title | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) |
|---|---|
| Place | Ireland |
| City | Dublin |
| Period | 9 - 13 October 2016 |
Link(s)
Abstract
We developed a novel circulating tumor cell (CTC) cluster microfluidic assay designed to mimic the in vivo tumor microenvironment for the label-free expansion of CTCs from clinically relevant blood volumes. Using specialized tapered microwells which promotes and mimics confined fluidic niche, we successfully expanded>50% of clinical blood samples (n=73) to achieve CTC clusters within two weeks. Putative CTCs may be presented throughout the cluster as single cells or aggregated microemboli, and could be detected with a panel of downstream analysis. The assay demonstrates superior performance in generating positive cultures over previously reported studies (<20%).
Research Area(s)
- Circulating tumor cells, CTC cluster, Drug response, Microfluidics, Microwells
Citation Format(s)
MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS. / Khoo, B.L.; Grenci, G.; Jing, T. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Vol. 1 Chemical and Biological Microsystems Society, 2016. p. 280-282.Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45) › 32_Refereed conference paper (with ISBN/ISSN) › peer-review
