A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Article numbere202200060
Journal / PublicationChemistry - An Asian Journal
Issue number12
Online published12 Apr 2022
Publication statusPublished - 15 Jun 2022


The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential ‘‘OFF-ON’’ fluorescence responsive probe for detecting hydrazine in vitro and in living cells.

Research Area(s)

  • covalent bonding, fluorescence quench, graphene oxide, hydrazine, resorufin