The effect of estradiol benzoate administration on estrous response and synchronized pregnancy rate in dairy heifers after treatment with exogenous progesterone

The objectives of this trial were to determine the effects of 0.5 mg estradiol benzoate administered intramuscularly 24 h after the removal of progesterone-containing intravaginal devices on the occurrence and timing of estrus, synchronized pregnancy rate and synchronized conception rate in dairy heifers. A clinical trial was conducted involving 750 dairy heifers in 13 herds. Within each herd heifers were randomly allocated to 1 of 2 estrus synchronization treatments. All heifers received a CIDR-B progesterone-containing intravaginal device containing a 10 mg estradiol benzoate capsule for 12 d. Twenty-four hours after CIDR-B removal one group received 0.5 mg, im estradiol benzoate while the other group received an intramuscular injection of a placebo. Estrus detection was performed at 48 and 72 h after intravaginal device removal, and heifers detected in estrus at those times were inseminated. Administration of estradiol benzoate 24 h after removal of CIDR-B devices significantly increased the number of heifers exhibiting estrus within the observation period (96.1 vs 90.5%; P