Protocol to induce neurodegeneration in a local area of the mouse brain by stereotaxic injection

Xuebing Zhang; Qingqing Lu; Xingqi Meng; Jin Young Kim

doi:10.1016/j.xpro.2024.103243

Protocol to induce neurodegeneration in a local area of the mouse brain by stereotaxic injection

Xuebing Zhang, Qingqing Lu, Xingqi Meng, Jin Young Kim^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

In vivo models of brain pathology are crucial for studying neurological diseases. Here, we present a protocol to induce a pathological condition in a mouse brain area by local injection of neurotoxic stimulus. We describe steps for preparing reagents, stereotaxic injection procedures to induce neurodegeneration in the hippocampus, and preparation of brain sections to examine the induced model. This protocol is useful for studying how local pathology affects other brain areas and neighbor cells and its functional consequences in behavior.

For complete details on the use and execution of this protocol, please refer to Zhang et al.

© 2024 The Author(s). Published by Elsevier Inc

Original language	English
Article number	103243
Journal	STAR Protocols
Volume	5
Issue number	3
Online published	2 Aug 2024
DOIs	https://doi.org/10.1016/j.xpro.2024.103243
Publication status	Published - 20 Sept 2024

Funding

This work was supported by grants from the Research Grants Council of the Hong Kong Special Administrative Region, China (project nos. CityU 11102220 and CityU 11101019 to J.Y.K.) and a grant from Tung Biomedical Sciences Centre at City University of Hong Kong (project no. 9609310 to J.Y.K.).

Research Keywords

Behavior
Cell Biology
Cell-based Assays
Model Organisms
Neuroscience

Publisher's Copyright Statement

This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/

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abstract = "In vivo models of brain pathology are crucial for studying neurological diseases. Here, we present a protocol to induce a pathological condition in a mouse brain area by local injection of neurotoxic stimulus. We describe steps for preparing reagents, stereotaxic injection procedures to induce neurodegeneration in the hippocampus, and preparation of brain sections to examine the induced model. This protocol is useful for studying how local pathology affects other brain areas and neighbor cells and its functional consequences in behavior. For complete details on the use and execution of this protocol, please refer to Zhang et al. {\textcopyright} 2024 The Author(s). Published by Elsevier Inc",

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AU - Zhang, Xuebing

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AU - Meng, Xingqi

AU - Kim, Jin Young

PY - 2024/9/20

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AB - In vivo models of brain pathology are crucial for studying neurological diseases. Here, we present a protocol to induce a pathological condition in a mouse brain area by local injection of neurotoxic stimulus. We describe steps for preparing reagents, stereotaxic injection procedures to induce neurodegeneration in the hippocampus, and preparation of brain sections to examine the induced model. This protocol is useful for studying how local pathology affects other brain areas and neighbor cells and its functional consequences in behavior. For complete details on the use and execution of this protocol, please refer to Zhang et al. © 2024 The Author(s). Published by Elsevier Inc

KW - Behavior

KW - Cell Biology

KW - Cell-based Assays

KW - Model Organisms

KW - Neuroscience

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DO - 10.1016/j.xpro.2024.103243

M3 - RGC 21 - Publication in refereed journal

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SN - 2666-1667

VL - 5

JO - STAR Protocols

JF - STAR Protocols

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ER -

Protocol to induce neurodegeneration in a local area of the mouse brain by stereotaxic injection

Abstract

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Research Keywords

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ASDC_Sub: Neural Cell Networking Mediated by Circadian Clocks to Enhance Brain Repair Systems

GRF: Inter-regional Communication Mechanisms Mediated by Circadian Clocks in Pathological Conditions of the Brain

GRF: The Circadian Clock-dependent Cell-fate Decision of Adult Neural Stem Cells: How the Circadian Clock Controls Demyelination/Remyelination in the Brain

Cite this

Protocol to induce neurodegeneration in a local area of the mouse brain by stereotaxic injection

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

ASDC_Sub: Neural Cell Networking Mediated by Circadian Clocks to Enhance Brain Repair Systems

GRF: Inter-regional Communication Mechanisms Mediated by Circadian Clocks in Pathological Conditions of the Brain

GRF: The Circadian Clock-dependent Cell-fate Decision of Adult Neural Stem Cells: How the Circadian Clock Controls Demyelination/Remyelination in the Brain

Cite this