Protocol for induction of heterosynaptic long-term potentiation in the mouse hippocampus via dual-opsin stimulation technique

Fengwen Huang; Stephen Temitayo Bello; Abdul Baset; Xi Chen; Jufang He

doi:10.1016/j.xpro.2024.102860

Protocol for induction of heterosynaptic long-term potentiation in the mouse hippocampus via dual-opsin stimulation technique

Fengwen Huang^*, Stephen Temitayo Bello, Abdul Baset, Xi Chen, Jufang He^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

1 Citation (Scopus)

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Abstract

Cholecystokinin (CCK) is the most abundant neuropeptide that broadly regulates the physiological status of animals. Here, we present a two-color laser theta burst stimulation (L-TBS) protocol for simultaneous activation of Schaffer collateral and perforant pathway in the hippocampus of CCK Cre mice. We describe steps for heterosynaptic long-term potentiation induction by L-TBS. This technique allows for the examination of the neurotransmitter roles in synaptic modulation and facilitates the exploration of pathological mechanisms in genetic models of brain disorders in mice.

For complete details on the use and execution of this protocol, please refer to Su et al.¹

Original language	English
Article number	102860
Journal	STAR Protocols
Volume	5
Issue number	1
Online published	1 Feb 2024
DOIs	https://doi.org/10.1016/j.xpro.2024.102860
Publication status	Published - 15 Mar 2024

Funding

This work was supported by Hong Kong Research Grants Council, General Research Fund (GRF): 11103220 (J.H.), 11101521 (J.H.), 11103922 (J.H.), and 11101818 (J.H.); Hong Kong Research Grants Council, Collaborative Research Fund (CRF): C1043-21G (J.H.); Hong Kong Research Grants Council, Theme-Based Research Scheme (TBRS): T13-605/18-W (J.H.); Innovation and Technology Fund (ITF): GHP_075_19GD (J.H.); Health and Medical Research Fund (HMRF): 09203656 (J.H.) and 06172456 (X.C.); and Guangdong Province Science and Technology Program ( 2020A0505140007 ). Cartoon figures were prepared using BioRender platform.

Research Keywords

Model Organisms
Neuroscience

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This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/

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abstract = "Cholecystokinin (CCK) is the most abundant neuropeptide that broadly regulates the physiological status of animals. Here, we present a two-color laser theta burst stimulation (L-TBS) protocol for simultaneous activation of Schaffer collateral and perforant pathway in the hippocampus of CCK Cre mice. We describe steps for heterosynaptic long-term potentiation induction by L-TBS. This technique allows for the examination of the neurotransmitter roles in synaptic modulation and facilitates the exploration of pathological mechanisms in genetic models of brain disorders in mice. For complete details on the use and execution of this protocol, please refer to Su et al.1{\textcopyright} 2024 The Author(s)",

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AU - Huang, Fengwen

AU - Bello, Stephen Temitayo

AU - Baset, Abdul

AU - Chen, Xi

AU - He, Jufang

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Cholecystokinin (CCK) is the most abundant neuropeptide that broadly regulates the physiological status of animals. Here, we present a two-color laser theta burst stimulation (L-TBS) protocol for simultaneous activation of Schaffer collateral and perforant pathway in the hippocampus of CCK Cre mice. We describe steps for heterosynaptic long-term potentiation induction by L-TBS. This technique allows for the examination of the neurotransmitter roles in synaptic modulation and facilitates the exploration of pathological mechanisms in genetic models of brain disorders in mice. For complete details on the use and execution of this protocol, please refer to Su et al.1© 2024 The Author(s)

AB - Cholecystokinin (CCK) is the most abundant neuropeptide that broadly regulates the physiological status of animals. Here, we present a two-color laser theta burst stimulation (L-TBS) protocol for simultaneous activation of Schaffer collateral and perforant pathway in the hippocampus of CCK Cre mice. We describe steps for heterosynaptic long-term potentiation induction by L-TBS. This technique allows for the examination of the neurotransmitter roles in synaptic modulation and facilitates the exploration of pathological mechanisms in genetic models of brain disorders in mice. For complete details on the use and execution of this protocol, please refer to Su et al.1© 2024 The Author(s)

KW - Model Organisms

KW - Neuroscience

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U2 - 10.1016/j.xpro.2024.102860

DO - 10.1016/j.xpro.2024.102860

M3 - RGC 21 - Publication in refereed journal

C2 - 38306268

AN - SCOPUS:85183958569

SN - 2666-1667

VL - 5

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 102860

ER -

Protocol for induction of heterosynaptic long-term potentiation in the mouse hippocampus via dual-opsin stimulation technique

Abstract

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GRF: Heterosynaptic Neuroplasticity of the Ascending Inputs in the Auditory Thalamus by Corticothalamic Modulation

HMRF: CCK-B Receptor Antagonist for the Treatment of Depression in the Mouse Model

CRF: Synthesized CCK-B Receptor Agonists to Alleviate Anterograde Amnesia in Animal Models

Cite this

Protocol for induction of heterosynaptic long-term potentiation in the mouse hippocampus via dual-opsin stimulation technique

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

GRF: Heterosynaptic Neuroplasticity of the Ascending Inputs in the Auditory Thalamus by Corticothalamic Modulation

HMRF: CCK-B Receptor Antagonist for the Treatment of Depression in the Mouse Model

CRF: Synthesized CCK-B Receptor Agonists to Alleviate Anterograde Amnesia in Animal Models

Cite this