Protein-assisted stretching and immobilization of DNA molecules in a microchannel

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Detail(s)

Original languageEnglish
Pages (from-to)2499-2504
Journal / PublicationNano Letters
Volume6
Issue number11
Publication statusPublished - Nov 2006
Externally publishedYes

Abstract

We demonstrate a novel technique we call "protein-assisted DNA Immobilization" (PADI), to immobilize and stretch, but not overstretch, DNA molecules inside a micro/nanochannel with limited surface interactions while maintaining continuous hydration at physiological pH. The biological activity of the immobilized DNA molecules is confirmed by digesting the DNA with restriction enzymes in the microchannel. Single-molecule transcription, which has stringent requirements on the immobilized DNA with respect to surface Interactions and stretched lengths, is also successfully demonstrated on DNA molecules immobilized by PADI. In addition to arraying DNA molecules for study of DNA-protein interactions, the immobilization method could be used to construct DNA-templated nanoelectronic devices. © 2006 American Chemical Society.

Citation Format(s)

Protein-assisted stretching and immobilization of DNA molecules in a microchannel. / Dukkipati, Venkat Ram; Kim, Ji Hoon; Pang, Stella W.; Larson, Ronald G.

In: Nano Letters, Vol. 6, No. 11, 11.2006, p. 2499-2504.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review