Programmable RNA manipulation in living cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

1 Scopus Citations
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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)4861–4867
Journal / PublicationCellular and Molecular Life Sciences
Volume76
Online published31 Jul 2019
Publication statusPublished - Dec 2019

Abstract

RNAs are responsible for mediating genetic information flow within the cell. RNA splicing, modification, trafficking, translation, and stability are all controlled at the transcript level. However, biological tools to study and manipulate them in a programmable fashion are currently limited. In this review, we summarize recent advances regarding available RNA-targeting systems discovered so far, including CRISPR-based technologies—Cas9 and Cas13, and programmable RNA-binding proteins—PUF and PPR. These tools allow transcript-specific manipulation in gene expression.

Research Area(s)

  • APEX2, bioID, Cas13, Cas9, CRISPR, PPR, PUF, RNA-binding proteins