Programmable RNA manipulation in living cells
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › Not applicable › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
| Original language | English |
|---|---|
| Pages (from-to) | 4861–4867 |
| Journal / Publication | Cellular and Molecular Life Sciences |
| Volume | 76 |
| Online published | 31 Jul 2019 |
| Publication status | Published - Dec 2019 |
Link(s)
Abstract
RNAs are responsible for mediating genetic information flow within the cell. RNA splicing, modification, trafficking, translation, and stability are all controlled at the transcript level. However, biological tools to study and manipulate them in a programmable fashion are currently limited. In this review, we summarize recent advances regarding available RNA-targeting systems discovered so far, including CRISPR-based technologies—Cas9 and Cas13, and programmable RNA-binding proteins—PUF and PPR. These tools allow transcript-specific manipulation in gene expression.
Research Area(s)
- APEX2, bioID, Cas13, Cas9, CRISPR, PPR, PUF, RNA-binding proteins
Citation Format(s)
Programmable RNA manipulation in living cells. / Pei, Yu; Lu, Mingxing.
In: Cellular and Molecular Life Sciences, Vol. 76, 12.2019, p. 4861–4867.Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › Not applicable › peer-review
