Programmable RNA manipulation in living cells
Related Research Unit(s)
|Journal / Publication||Cellular and Molecular Life Sciences|
|Online published||31 Jul 2019|
|Publication status||Published - Dec 2019|
|Link to Scopus||https://www.scopus.com/record/display.uri?eid=2-s2.0-85069923571&origin=recordpage|
RNAs are responsible for mediating genetic information flow within the cell. RNA splicing, modification, trafficking, translation, and stability are all controlled at the transcript level. However, biological tools to study and manipulate them in a programmable fashion are currently limited. In this review, we summarize recent advances regarding available RNA-targeting systems discovered so far, including CRISPR-based technologies—Cas9 and Cas13, and programmable RNA-binding proteins—PUF and PPR. These tools allow transcript-specific manipulation in gene expression.
- APEX2, bioID, Cas13, Cas9, CRISPR, PPR, PUF, RNA-binding proteins