Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients

Mengyao Wang; Peng Gao; Laifeng Ren; Jingjing Duan; Silu Yang; Haina Wang; Hongxia Wang; Junning Sun; Xiaoyan Gao; Bo Li; Shuaicheng Li; Wen Su

doi:10.3389/fimmu.2022.848113

Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients

Mengyao Wang (Co-first Author), Peng Gao (Co-first Author), Laifeng Ren (Co-first Author), Jingjing Duan, Silu Yang, Haina Wang, Hongxia Wang, Junning Sun, Xiaoyan Gao, Bo Li, Shuaicheng Li^*, Wen Su^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

6 Citations (Scopus)

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Abstract

Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy.

Original language	English
Article number	848113
Number of pages	9
Journal	Frontiers in Immunology
Volume	13
Online published	28 Jul 2022
DOIs	https://doi.org/10.3389/fimmu.2022.848113
Publication status	Published - 28 Jul 2022

Research Keywords

TCR repertoire analysis
gastric cancer
TCR repertoire
next-generation sequencing
biomarker
antitumor immunity

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients",

abstract = "Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy.",

keywords = "TCR repertoire analysis, gastric cancer, TCR repertoire, next-generation sequencing, biomarker, antitumor immunity",

author = "Mengyao Wang and Peng Gao and Laifeng Ren and Jingjing Duan and Silu Yang and Haina Wang and Hongxia Wang and Junning Sun and Xiaoyan Gao and Bo Li and Shuaicheng Li and Wen Su",

year = "2022",

month = jul,

day = "28",

doi = "10.3389/fimmu.2022.848113",

language = "English",

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journal = "Frontiers in Immunology",

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TY - JOUR

T1 - Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients

AU - Wang, Mengyao

AU - Gao, Peng

AU - Ren, Laifeng

AU - Duan, Jingjing

AU - Yang, Silu

AU - Wang, Haina

AU - Wang, Hongxia

AU - Sun, Junning

AU - Gao, Xiaoyan

AU - Li, Bo

AU - Li, Shuaicheng

AU - Su, Wen

PY - 2022/7/28

Y1 - 2022/7/28

N2 - Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy.

AB - Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy.

KW - TCR repertoire analysis

KW - gastric cancer

KW - TCR repertoire

KW - next-generation sequencing

KW - biomarker

KW - antitumor immunity

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U2 - 10.3389/fimmu.2022.848113

DO - 10.3389/fimmu.2022.848113

M3 - RGC 21 - Publication in refereed journal

C2 - 35967453

SN - 1664-3224

VL - 13

JO - Frontiers in Immunology

JF - Frontiers in Immunology

M1 - 848113

ER -

Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients

Abstract

Research Keywords

Publisher's Copyright Statement

UN SDGs

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