Abstract
The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21. mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20. min) and 99.5% (40. min) at 850. mbar and 25. °C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450. mbar, 35. °C and 40. min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8. days, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 500-505 |
| Journal | Bioresource Technology |
| Volume | 200 |
| DOIs | |
| Publication status | Published - 1 Jan 2016 |
| Externally published | Yes |
Bibliographical note
Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].Research Keywords
- Chlorella sorokiniana
- Lutein
- Microalgae
- Reduced pressure extraction
- Stabilization