Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers

Youhua Tan; Chi-wing Kong; Shuxun Chen; Shuk Han Cheng; Ronald A. Li; Dong Sun

doi:10.1016/j.jbiomech.2011.09.007

Author(s)

Youhua Tan
Chi-wing Kong
Shuxun Chen
Ronald A. Li

Detail(s)

Original language	English
Pages (from-to)	123-128
Journal / Publication	Journal of Biomechanics
Volume	45
Issue number	1
Publication status	Published - 3 Jan 2012

Link(s)

DOI	DOI https://doi.org/10.1016/j.jbiomech.2011.09.007 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-83555178421&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(b964dc6a-9e7a-4383-89c9-af6da4b3a7ea).html

Abstract

Human embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and static mechanical responses of cells during optical stretching. Our experiments showed that the mechanical stiffness of differentiated hESC-CM increased after cardiac differentiation. Such stiffening could associate with increasingly organized myofibrillar assembly that underlines the functional characteristics of hESC-CM. In summary, our findings lay the ground work for using hESC-CMs as models to study mechanical and contractile defects in heart diseases. © 2011 Elsevier Ltd.

Research Area(s)

Biomechanics, Cardiac differentiation, Cell manipulation, Human embryonic stem cell, Optical tweezers

Citation Format(s)

[ RIS ] [ BibTeX ]

Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers. / Tan, Youhua; Kong, Chi-wing; Chen, Shuxun et al.

In: Journal of Biomechanics, Vol. 45, No. 1, 03.01.2012, p. 123-128.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Tan, Y, Kong, C, Chen, S, Cheng, SH, Li, RA & Sun, D 2012, 'Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers', Journal of Biomechanics, vol. 45, no. 1, pp. 123-128. https://doi.org/10.1016/j.jbiomech.2011.09.007

Tan, Y., Kong, C., Chen, S., Cheng, S. H., Li, R. A., & Sun, D. (2012). Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers. Journal of Biomechanics, 45(1), 123-128. https://doi.org/10.1016/j.jbiomech.2011.09.007

Tan Y, Kong C, Chen S, Cheng SH, Li RA, Sun D. Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers. Journal of Biomechanics. 2012 Jan 3;45(1):123-128. doi: 10.1016/j.jbiomech.2011.09.007

Tan, Youhua ; Kong, Chi-wing ; Chen, Shuxun et al. / Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers. In: Journal of Biomechanics. 2012 ; Vol. 45, No. 1. pp. 123-128.