Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers

Kaiqun Wang; Jinping Cheng; Shuk Han Cheng; Dong Sun

doi:10.1063/1.4819392

Author(s)

Kaiqun Wang
Jinping Cheng
Shuk Han Cheng
Dong Sun

Detail(s)

Original language	English
Article number	083706
Journal / Publication	Applied Physics Letters
Volume	103
Issue number	8
Online published	23 Aug 2013
Publication status	Published - Aug 2013

Link(s)

DOI	DOI https://doi.org/10.1063/1.4819392 Final Published version
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Attachment(s)	Documents 5870085.pdf Final Published version, 1.09 MB, PDF Publisher's Copyright Statement This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Kaiqun Wang, Jinping Cheng, Shuk Han Cheng, and Dong Sun , "Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers", Appl. Phys. Lett. 103, 083706 (2013) and may be found at https://doi.org/10.1063/1.4819392.
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84883383057&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(675dbb64-0f33-4454-ac04-01541de0b92e).html

Abstract

This letter presents an approach to utilizing the actin cytoskeleton model and optical tweezers technology to probe the distinct underlying F-actin remodeling mechanism and showing quantitatively how cell mechanical behavior is associated with alterations in the cell functions. The structural parameters of F-actin were extracted by fitting the modeling results with the experimental results obtained by cell stretching manipulation. Alterations of cell mechanical behaviors under distinct diseased cellular stages were further interpreted. Jurkat and K562 cells were used as sample cells. This letter successfully illustrates the correlation of the cell mechanical behavior and cell functional alterations in a quantitative way. © 2013 AIP Publishing LLC.