Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers

Kaiqun Wang; Jinping Cheng; Shuk Han Cheng; Dong Sun

doi:10.1063/1.4819392

Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers

Kaiqun Wang
, Jinping Cheng
, Shuk Han Cheng
, Dong Sun^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

22 Citations (Scopus)

42 Downloads (CityUHK Scholars)

Abstract

This letter presents an approach to utilizing the actin cytoskeleton model and optical tweezers technology to probe the distinct underlying F-actin remodeling mechanism and showing quantitatively how cell mechanical behavior is associated with alterations in the cell functions. The structural parameters of F-actin were extracted by fitting the modeling results with the experimental results obtained by cell stretching manipulation. Alterations of cell mechanical behaviors under distinct diseased cellular stages were further interpreted. Jurkat and K562 cells were used as sample cells. This letter successfully illustrates the correlation of the cell mechanical behavior and cell functional alterations in a quantitative way. © 2013 AIP Publishing LLC.

Original language	English
Article number	083706
Journal	Applied Physics Letters
Volume	103
Issue number	8
Online published	23 Aug 2013
DOIs	https://doi.org/10.1063/1.4819392
Publication status	Published - Aug 2013

Publisher's Copyright Statement

COPYRIGHT TERMS OF DEPOSITED FINAL PUBLISHED VERSION FILE: This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Kaiqun Wang, Jinping Cheng, Shuk Han Cheng, and Dong Sun , "Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers", Appl. Phys. Lett. 103, 083706 (2013) and may be found at https://doi.org/10.1063/1.4819392.

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title = "Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers",

abstract = "This letter presents an approach to utilizing the actin cytoskeleton model and optical tweezers technology to probe the distinct underlying F-actin remodeling mechanism and showing quantitatively how cell mechanical behavior is associated with alterations in the cell functions. The structural parameters of F-actin were extracted by fitting the modeling results with the experimental results obtained by cell stretching manipulation. Alterations of cell mechanical behaviors under distinct diseased cellular stages were further interpreted. Jurkat and K562 cells were used as sample cells. This letter successfully illustrates the correlation of the cell mechanical behavior and cell functional alterations in a quantitative way. {\textcopyright} 2013 AIP Publishing LLC.",

author = "Kaiqun Wang and Jinping Cheng and \{Han Cheng\}, Shuk and Dong Sun",

year = "2013",

month = aug,

doi = "10.1063/1.4819392",

language = "English",

volume = "103",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics",

number = "8",

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T1 - Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers

AU - Wang, Kaiqun

AU - Cheng, Jinping

AU - Han Cheng, Shuk

AU - Sun, Dong

PY - 2013/8

Y1 - 2013/8

N2 - This letter presents an approach to utilizing the actin cytoskeleton model and optical tweezers technology to probe the distinct underlying F-actin remodeling mechanism and showing quantitatively how cell mechanical behavior is associated with alterations in the cell functions. The structural parameters of F-actin were extracted by fitting the modeling results with the experimental results obtained by cell stretching manipulation. Alterations of cell mechanical behaviors under distinct diseased cellular stages were further interpreted. Jurkat and K562 cells were used as sample cells. This letter successfully illustrates the correlation of the cell mechanical behavior and cell functional alterations in a quantitative way. © 2013 AIP Publishing LLC.

AB - This letter presents an approach to utilizing the actin cytoskeleton model and optical tweezers technology to probe the distinct underlying F-actin remodeling mechanism and showing quantitatively how cell mechanical behavior is associated with alterations in the cell functions. The structural parameters of F-actin were extracted by fitting the modeling results with the experimental results obtained by cell stretching manipulation. Alterations of cell mechanical behaviors under distinct diseased cellular stages were further interpreted. Jurkat and K562 cells were used as sample cells. This letter successfully illustrates the correlation of the cell mechanical behavior and cell functional alterations in a quantitative way. © 2013 AIP Publishing LLC.

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84883383057&origin=recordpage

U2 - 10.1063/1.4819392

DO - 10.1063/1.4819392

M3 - RGC 21 - Publication in refereed journal

SN - 0003-6951

VL - 103

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 8

M1 - 083706

ER -

Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers

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CRF: Development of Cell Manipulation Tools for Probing Functional Mechanism of Hematopoietic Cells: Robotics, Optical tweezers, and Hematopoiesis

CRF: Development of Cell Manipulation Tools for Probing Functional Mechanism of Hematopoietic Cells: Robotics, Optical tweezers, and Hematopoiesis

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Probing cell biophysical behavior based on actin cytoskeleton modeling and stretching manipulation with optical tweezers

Abstract

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CRF: Development of Cell Manipulation Tools for Probing Functional Mechanism of Hematopoietic Cells: Robotics, Optical tweezers, and Hematopoiesis

CRF: Development of Cell Manipulation Tools for Probing Functional Mechanism of Hematopoietic Cells: Robotics, Optical tweezers, and Hematopoiesis

Cite this