Prevalence of Asymptomatic Urinary Shedding of Pathogenic Leptospires in Asymptomatic Cats in Hong Kong

Research output: Conference PapersPosterpeer-review

View graph of relations

Author(s)

  • Francesca Rizzo
  • Lok See Tiffany Cheng
  • Yen Yeen Chor
  • Yingfei Chai
  • Yan Ru Choi
  • Fiona Woodhouse
  • Jane J. Gray

Detail(s)

Original languageEnglish
Publication statusPublished - Sept 2022

Conference

Title6th International Society for Companion Animal Infectious Diseases Symposium (ISCAID 2022)
PlaceUnited Kingdom
CityGlasgow
Period4 - 7 September 2022

Abstract

BACKGROUND
Leptospirosis is a worldwide bacterial zoonosis. Cats can be asymptomatically infected and shed Leptospira in urine.

A
IMS
To determine the prevalence and associated risk factors for Leptospira DNA in urine of unowned cats in Hong Kong (HK).

M
ETHODS
Urine samples were collected from free-roaming unowned cats at a trap-neuter-release clinic. Presence of amplifiable DNA was confirmed after extraction from urine, using a GAPDH PCR. Pathogenic leptospires were detected with a qPCR assay targeting the Lip32 gene, including standard 10-fold dilutions of a Lip32 plasmid construct from 108–101 copies/reaction to allow target quantification and evaluate qPCR efficiency. Location trapped was classified by 9 districts in New Territories (NT), 5 in Kowloon (KLN) and 4 in Hong Kong Island (HKI). Risk factors including age, sex and rodent infestation rate (RIR) were analysed by Chi-squared tests and logistic regression.

R
ESULTS
Urine samples were collected from 198 cats (98 females, 100 males), median age 2 years (range 0.4–12). In total 11 (5.56%) cats (4 females,7 males), median age 3 years, tested positive for Leptospira DNA including 2/25 (8%) from HKI, 2/30 (6.67%) from KLN and 7/143 from NT (4.9%). The mean and median number of copies detected was 5.77×103 and 9.32×101, respectively (range 4.37–3.01 ×104) per 10 μL/template. No risk factors were significantly associated with urinary shedding.

D
ISCUSSION/ CONCLUSIONS
This data confirms the circulation of pathogenic Leptospira species in stray cats, at a low prevalence in all three major regions of HK.

Citation Format(s)

Prevalence of Asymptomatic Urinary Shedding of Pathogenic Leptospires in Asymptomatic Cats in Hong Kong. / Rizzo, Francesca; Cheng, Lok See Tiffany; Chor, Yen Yeen et al.
2022. Poster session presented at 6th International Society for Companion Animal Infectious Diseases Symposium (ISCAID 2022), Glasgow, United Kingdom.

Research output: Conference PapersPosterpeer-review