Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)164-168
Journal / PublicationMarine Pollution Bulletin
Volume64
Issue number1
Publication statusPublished - Jan 2012

Abstract

Marine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. © 2011 Elsevier Ltd.

Research Area(s)

  • Fecal contamination, Hong Kong marine waters, Noroviruses, NoV GI.1, NoV GII.4, TaqMan real-time PCR

Citation Format(s)

Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR. / Yang, Ning; Qi, Huizhou; Wong, Minnie Man Lai; Wu, Rudolf Shiu Sun; Kong, Richard Yuen Chong.

In: Marine Pollution Bulletin, Vol. 64, No. 1, 01.2012, p. 164-168.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review