Prevalence and dissemination of oqxAB in Escherichia coli isolates from animals, farmworkers, and the environment

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

114 Scopus Citations
View graph of relations

Author(s)

  • Jingjing Zhao
  • Zhangliu Chen
  • Yuting Deng
  • Yahong Liu
  • Wei Tian
  • Xianhui Huang
  • Congming Wu
  • Yongxu Sun
  • Yan Sun
  • Zhenling Zeng
  • Jian-Hua Liu

Detail(s)

Original languageEnglish
Pages (from-to)4219-4224
Journal / PublicationAntimicrobial Agents and Chemotherapy
Volume54
Issue number10
Online published17 Sep 2010
Publication statusPublished - Oct 2010
Externally publishedYes

Abstract

OqxAB has recently been identified as one of the mechanisms of plasmid-mediated quinolone resistance (PMQR). Compared to what is observed for other PMQR determinants, there is a paucity of data with regard to the prevalence and epidemiology of OqxAB and its contribution to resistance to different antimicrobials. In this study, the prevalence and dissemination of oqxAB and other PMQR genes in Escherichia coli isolates from animals, farmworkers, and the environment in 2002 in China were investigated. Of the 172 E. coli isolates, 39.0% carried oqxA, while only 4.1%, 2.9%, and 0.6% carried qnr (1 qnrB6 isolate, 5 qnrS1 isolates, and 1 qnrD isolate), qepA, and aac(6′)-Ib-cr, respectively. Among the 33 isolates from farmworkers, 10 (30.3%) were positive for oqxA. oqxAB was associated with IS26 and was carried on the 43- to 115-kb IncF transferable plasmid. Transconjugants carrying oqxAB showed 4- to 16-fold increases in the MICs of quinolones, 16- to 64-fold increases in the MICs of quinoxalines, 8- to 32-fold increases in the MICs of chloramphenicol and trimethoprim-sulfamethoxazole, and 4- to 8-fold increases in the MICs of florfenicol compared to the levels for the recipient. The pulsed-field gel electrophoresis (PFGE) analysis showed that the high levels of prevalence and dissemination of oqxAB in E. coli in animal farms were primarily due to the transmission of plasmids carrying oqxAB, although clonal transmission between human and swine E. coli isolates was observed. It is concluded that oqxAB was widespread in animal farms in China, which may be due to the overuse of quinoxalines in animals. This study warrants the prudent use of quinoxalines in food animals. 

Citation Format(s)

Prevalence and dissemination of oqxAB in Escherichia coli isolates from animals, farmworkers, and the environment. / Zhao, Jingjing; Chen, Zhangliu; Chen, Sheng; Deng, Yuting; Liu, Yahong; Tian, Wei; Huang, Xianhui; Wu, Congming; Sun, Yongxu; Sun, Yan; Zeng, Zhenling; Liu, Jian-Hua.

In: Antimicrobial Agents and Chemotherapy, Vol. 54, No. 10, 10.2010, p. 4219-4224.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal