Preparation of small-molecule microarrays by trans-cyclooctene tetrazine ligation and their application in the high-throughput screening of protein-protein interaction inhibitors of bromodomains

Chong-Jing Zhang; Chelsea Y. J. Tan; Jingyan Ge; Zhenkun Na; Grace Y. J. Chen; Mahesh Uttamchandani; Hongyan Sun; Shao Q. Yao

doi:10.1002/anie.201307803

Author(s)

Chong-Jing Zhang
Chelsea Y. J. Tan
Jingyan Ge
Zhenkun Na
Grace Y. J. Chen
Mahesh Uttamchandani
Shao Q. Yao

Detail(s)

Original language	English
Pages (from-to)	14060-14064
Journal / Publication	Angewandte Chemie - International Edition
Volume	52
Issue number	52
Online published	4 Nov 2013
Publication status	Published - 23 Dec 2013

Link(s)

DOI	DOI https://doi.org/10.1002/anie.201307803 Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84890587427&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(bff0091b-82f5-452a-966b-d7d943b6b990).html

Abstract

Fast and efficient: A library of trans-cyclooctene (TCO)-modified small molecules were immobilized on tetrazine-functionalized glass slides by using the fastest bioorthogonal reaction known. The resulting small-molecule microarray was screened against a variety of human bromodomains to identify protein-protein interaction inhibitors. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Research Area(s)

bioorthogonal chemistry, epigenetics, inhibitors, protein-protein interactions, small-molecule microarrays

Citation Format(s)

[ RIS ] [ BibTeX ]

Preparation of small-molecule microarrays by trans-cyclooctene tetrazine ligation and their application in the high-throughput screening of protein-protein interaction inhibitors of bromodomains. / Zhang, Chong-Jing; Tan, Chelsea Y. J.; Ge, Jingyan et al.

In: Angewandte Chemie - International Edition, Vol. 52, No. 52, 23.12.2013, p. 14060-14064.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Zhang, C-J, Tan, CYJ, Ge, J, Na, Z, Chen, GYJ, Uttamchandani, M, Sun, H & Yao, SQ 2013, 'Preparation of small-molecule microarrays by trans-cyclooctene tetrazine ligation and their application in the high-throughput screening of protein-protein interaction inhibitors of bromodomains', Angewandte Chemie - International Edition, vol. 52, no. 52, pp. 14060-14064. https://doi.org/10.1002/anie.201307803

Zhang, C-J., Tan, C. Y. J., Ge, J., Na, Z., Chen, G. Y. J., Uttamchandani, M., Sun, H., & Yao, S. Q. (2013). Preparation of small-molecule microarrays by trans-cyclooctene tetrazine ligation and their application in the high-throughput screening of protein-protein interaction inhibitors of bromodomains. Angewandte Chemie - International Edition, 52(52), 14060-14064. https://doi.org/10.1002/anie.201307803

Zhang C-J, Tan CYJ, Ge J, Na Z, Chen GYJ, Uttamchandani M et al. Preparation of small-molecule microarrays by trans-cyclooctene tetrazine ligation and their application in the high-throughput screening of protein-protein interaction inhibitors of bromodomains. Angewandte Chemie - International Edition. 2013 Dec 23;52(52):14060-14064. Epub 2013 Nov 4. doi: 10.1002/anie.201307803

Zhang, Chong-Jing ; Tan, Chelsea Y. J. ; Ge, Jingyan et al. / Preparation of small-molecule microarrays by trans-cyclooctene tetrazine ligation and their application in the high-throughput screening of protein-protein interaction inhibitors of bromodomains. In: Angewandte Chemie - International Edition. 2013 ; Vol. 52, No. 52. pp. 14060-14064.