Preparation of biodiesel from nonedible oils using a mixture of used lipases

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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  • Sanjib Kumar Karmee

Related Research Unit(s)


Original languageEnglish
Pages (from-to)2727-2733
Journal / PublicationEnergy Sources, Part A: Recovery, Utilization and Environmental Effects
Issue number18
Publication statusPublished - 16 Sep 2016


Biodiesel was synthesized from nonedible oils using a lipase mixture composed of used and discarded Candida rugusa, Candida antactica-B (Novozyme-435), Pseudomonas cepacia, Rhizopus oryzae, and porcine pancreas Type II lipase. To avoid the lipase deactivation stepwise addition of 6 mmol of methanol to 1 mmol of oil lead to 93% biodiesel yield. Addition of 10 wt% of silica gel to the reaction mixture resulted in 97% biodiesel. The lipase mixture was recycled for five times and at the end of the fifth cycle 86% biodiesel was formed.

Research Area(s)

  • Biodiesel, lipase mixture