Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice

Alessandra M.M.G. de Castro; Taís F. Cruz; Katarina B. Yamada; Priscilla F. Gerber; Michelle P. Gabardo; João P. Araújo; Roberto M.C. Guedes; Cinthia K. Mori; Camila P. Oliveira; Sueli S. Santos; Leonardo J. Richtzenhain

doi:10.1016/j.rvsc.2015.09.008

Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice

Alessandra M.M.G. de Castro^*
, Taís F. Cruz
, Katarina B. Yamada
, Priscilla F. Gerber
, Michelle P. Gabardo
, João P. Araújo
, Roberto M.C. Guedes
, Cinthia K. Mori
, Camila P. Oliveira
, Sueli S. Santos
, Leonardo J. Richtzenhain

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

7 Citations (Scopus)

Abstract

Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice.

Original language	English
Pages (from-to)	70-72
Journal	Research in Veterinary Science
Volume	103
DOIs	https://doi.org/10.1016/j.rvsc.2015.09.008
Publication status	Published - 1 Dec 2015
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]

Funding

The authors would like to thank the São Paulo Research Foundation (FAPESP), São Paulo State, Brazil (grant number 2007/57115-3), for financial support. The authors Guedes and Mori (grant number 2010-2899) would like to thank the Conselho Nacional de Desenvolvimento Científico (CNPq) for research fellowships. The authors Yamada (grant number 2011/00054-8) and Castro (grant number 2009/09175-2) would like to thank FAPESP for research fellowships.

Research Keywords

PCV2
Rodents
Swine virus
Viral load

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10.1016/j.rvsc.2015.09.008

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de Castro, A. M. M. G., Cruz, T. F., Yamada, K. B., Gerber, P. F., Gabardo, M. P., Araújo, J. P., Guedes, R. M. C., Mori, C. K., Oliveira, C. P., Santos, S. S., & Richtzenhain, L. J. (2015). Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice. Research in Veterinary Science, 103, 70-72. https://doi.org/10.1016/j.rvsc.2015.09.008

@article{ae13c4d1bf66403398d57175b772dc61,

title = "Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice",

abstract = "Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice.",

keywords = "PCV2, Rodents, Swine virus, Viral load",

author = "\{de Castro\}, \{Alessandra M.M.G.\} and Cruz, \{Ta{\'i}s F.\} and Yamada, \{Katarina B.\} and Gerber, \{Priscilla F.\} and Gabardo, \{Michelle P.\} and Ara{\'u}jo, \{Jo{\~a}o P.\} and Guedes, \{Roberto M.C.\} and Mori, \{Cinthia K.\} and Oliveira, \{Camila P.\} and Santos, \{Sueli S.\} and Richtzenhain, \{Leonardo J.\}",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]",

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doi = "10.1016/j.rvsc.2015.09.008",

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de Castro, AMMG, Cruz, TF, Yamada, KB, Gerber, PF, Gabardo, MP, Araújo, JP, Guedes, RMC, Mori, CK, Oliveira, CP, Santos, SS & Richtzenhain, LJ 2015, 'Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice', Research in Veterinary Science, vol. 103, pp. 70-72. https://doi.org/10.1016/j.rvsc.2015.09.008

Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice. / de Castro, Alessandra M.M.G.; Cruz, Taís F.; Yamada, Katarina B. et al.
In: Research in Veterinary Science, Vol. 103, 01.12.2015, p. 70-72.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice

AU - de Castro, Alessandra M.M.G.

AU - Cruz, Taís F.

AU - Yamada, Katarina B.

AU - Gerber, Priscilla F.

AU - Gabardo, Michelle P.

AU - Araújo, João P.

AU - Guedes, Roberto M.C.

AU - Mori, Cinthia K.

AU - Oliveira, Camila P.

AU - Santos, Sueli S.

AU - Richtzenhain, Leonardo J.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice.

AB - Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice.

KW - PCV2

KW - Rodents

KW - Swine virus

KW - Viral load

UR - https://www.scopus.com/pages/publications/84949563961

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84949563961&origin=recordpage

U2 - 10.1016/j.rvsc.2015.09.008

DO - 10.1016/j.rvsc.2015.09.008

M3 - RGC 21 - Publication in refereed journal

C2 - 26679798

SN - 0034-5288

VL - 103

SP - 70

EP - 72

JO - Research in Veterinary Science

JF - Research in Veterinary Science

ER -

Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice

Abstract

Bibliographical note

Funding

Research Keywords

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