Predominance of a macrolide-lincosamide-resistant Brachyspira hyodysenteriae of sequence type 196 in Swiss pig herds

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Ana B. García-Martín
  • Vincent Perreten
  • Alexandra Rossano
  • Sarah Schmitt
  • Heiko Nathues

Detail(s)

Original languageEnglish
Pages (from-to)97-102
Journal / PublicationVeterinary Microbiology
Volume226
Online published11 Oct 2018
Publication statusPublished - Nov 2018
Externally publishedYes

Abstract

Worldwide emergence of antimicrobial-resistant Brachyspira (B.) hyodysenteriae led us question whether specific clones are present in Switzerland. Fifty-one B. hyodysenteriae isolates originating from 27 different Swiss pig herds sampled between 2010 and 2017 were characterised. Multilocus sequence typing revealed the presence of four different sequence types (STs) ST6, ST66, ST196 and ST197 with ST196 being predominant. Antimicrobial susceptibility to six different antimicrobial agents was determined by measurement of the minimal inhibitory concentration by broth dilution. Isolates were examined for the presence of point mutations and genes known to be associated with antimicrobial resistance in B. hyodysenteriae by PCR and sequence analysis. Forty-one isolates belonging to ST6 (n = 1), ST66 (n = 4) and ST196 (n = 36) exhibited decreased susceptibility to macrolides and lincomycin associated with an A2058 T/G mutation in the 23S rRNA gene. One isolate of ST66 and five isolates of ST196 exhibited decreased susceptibility to doxycycline associated with a G1058C mutation in the 16S rRNA gene. The Swiss B. hyodysenteriae population is characterised by a low genetic diversity, with macrolide-lincosamide-resistant isolates of ST196 being predominant.

Research Area(s)

  • Antibiotic resistance, Antimicrobial susceptibility, Genotyping, Minimal inhibitory concentration, Swine dysentery

Citation Format(s)

Predominance of a macrolide-lincosamide-resistant Brachyspira hyodysenteriae of sequence type 196 in Swiss pig herds. / García-Martín, Ana B.; Perreten, Vincent; Rossano, Alexandra; Schmitt, Sarah; Nathues, Heiko; Zeeh, Friederike.

In: Veterinary Microbiology, Vol. 226, 11.2018, p. 97-102.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review