Precursor ion scan driven fast untargeted screening and semi-determination of caffeoylquinic acid derivatives in Cynara scolymus L.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Pages (from-to) | 442-447 |
Journal / Publication | Food Chemistry |
Volume | 166 |
Online published | 14 Jun 2014 |
Publication status | Published - 1 Jan 2015 |
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Abstract
A precursor ion scan (PIS) technique based strategy was developed for rapid screening and semi-determination of caffeoylquinic acid derivatives (CADs) in artichoke (Cynara scolymus L.) using ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry. 1,5-Dicaffeoylquinic acid and 5-caffeoylquinic acid were used for studying the fragmentation behaviour of two classes of CADs, setting m/z 191 as a diagnostic moiety. When it was applied to artichoke sample, ten CADs were detected and elucidated in a single PIS run. Furthermore, method validation was implemented including: specificity (no interference), linearity (≥0.9993), limit of detection (LOD <0.12 ng mL-1) and limit of quantification (LOQ <0.25 ng mL-1), precision (RSD ≤ 3.6), recovery (91.4-95.9%) and stability (at least 12 h). This approach was proven to be a powerful, selective and sensitive tool for rapid screening and semi-determination of untargeted components in natural products. © 2014 Elsevier Ltd. All rights reserved.
Research Area(s)
- Caffeoylquinic acid derivative, Cynara scolymus L, Precursor ion scan, UPLC-MS/MS
Citation Format(s)
Precursor ion scan driven fast untargeted screening and semi-determination of caffeoylquinic acid derivatives in Cynara scolymus L. / Shen, Qing; Lu, Yanbin; Dai, Zhiyuan et al.
In: Food Chemistry, Vol. 166, 01.01.2015, p. 442-447.
In: Food Chemistry, Vol. 166, 01.01.2015, p. 442-447.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review