Precursor ion scan driven fast untargeted screening and semi-determination of caffeoylquinic acid derivatives in Cynara scolymus L.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

12 Scopus Citations
View graph of relations

Author(s)

  • Qing Shen
  • Yanbin Lu
  • Zhiyuan Dai
  • Hon-Yeung Cheung

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)442-447
Journal / PublicationFood Chemistry
Volume166
Online published14 Jun 2014
Publication statusPublished - 1 Jan 2015

Abstract

A precursor ion scan (PIS) technique based strategy was developed for rapid screening and semi-determination of caffeoylquinic acid derivatives (CADs) in artichoke (Cynara scolymus L.) using ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry. 1,5-Dicaffeoylquinic acid and 5-caffeoylquinic acid were used for studying the fragmentation behaviour of two classes of CADs, setting m/z 191 as a diagnostic moiety. When it was applied to artichoke sample, ten CADs were detected and elucidated in a single PIS run. Furthermore, method validation was implemented including: specificity (no interference), linearity (≥0.9993), limit of detection (LOD <0.12 ng mL-1) and limit of quantification (LOQ <0.25 ng mL-1), precision (RSD ≤ 3.6), recovery (91.4-95.9%) and stability (at least 12 h). This approach was proven to be a powerful, selective and sensitive tool for rapid screening and semi-determination of untargeted components in natural products. © 2014 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Caffeoylquinic acid derivative, Cynara scolymus L, Precursor ion scan, UPLC-MS/MS