Poly(methyl methacrylate) Surface Modification for Surfactant-Free Real-Time Toxicity Assay on Droplet Microfluidic Platform

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Pages (from-to)13801-13811
Journal / PublicationACS Applied Materials and Interfaces
Volume9
Issue number15
Publication statusPublished - 19 Apr 2017
Externally publishedYes

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Abstract

Microfluidic droplet reactors have many potential uses, from analytical to synthesis. Stable operation requires preferential wetting of the channel surface by the continuous phase which is often not fulfilled by materials commonly used for lab-on-chip devices. Here we show that a silica nanoparticle (SiNP) layer coated onto a Poly(methyl methacrylate) (PMMA) and other thermoplastics surface enhances its wetting properties by creating nanoroughness, and allows simple grafting of hydrocarbon chains through silane chemistry. Using the unusual stability of silica sols at their isoelectric point, a dense SiNP layer is adsorbed onto PMMA and renders the surface superhydrophilic. Subsequently, a self-assembled dodecyltrichlorosilane (DTS) monolayer yields a superhydrophobic surface that allows the repeatable generation of aqueous droplets in a hexadecane continuous phase without surfactant addition. A SiNP-DTS modified chip has been used to monitor bacterial viability with a resazurin assay. The whole process involving sequential reagents injection, and multiplexed droplet fluorescence intensity monitoring is carried out on chip. Metabolic inhibition of the anaerobe Enterococcus faecalis by 30 mg L-1 of NiCl2 was detected in 5 min. © 2017 American Chemical Society

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Poly(methyl methacrylate) Surface Modification for Surfactant-Free Real-Time Toxicity Assay on Droplet Microfluidic Platform. / Ortiz, Raphael; CHEN, Jian Lin; Stuckey, David C.; Steele, Terry W.J.

In: ACS Applied Materials and Interfaces, Vol. 9, No. 15, 19.04.2017, p. 13801-13811.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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