Poly(lactide-co-glycolide)/titania composite microsphere-sintered scaffolds for bone tissue engineering applications

Yingjun Wang; Xuetao Shi; Li Ren; Yongchang Yao; Feng Zhang; Dong-An Wang

doi:10.1002/jbm.b.31561

Poly(lactide-co-glycolide)/titania composite microsphere-sintered scaffolds for bone tissue engineering applications

Yingjun Wang^*
, Xuetao Shi
, Li Ren
, Yongchang Yao
, Feng Zhang
, Dong-An Wang^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

41 Citations (Scopus)

Abstract

The objective of this study was to synthesize and characterize novel threedimensional porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-TiO₂-particle composite microspheres for potential bone repair applications. The introduction of TiO₂ component has been proven capable of largely enhancing mechanical properties of PLGA/ TiO ₂ microsphere-sintered scaffold ("PLGA/TiO₂- SMS"). In addition, composite nano-TiO₂ additives are capable of inducing an increased arrest of adhesive proteins from the environment, which benefits cell attachment onto the scaffolds. Osteoblast proliferation and maturation were evaluated by MTT assay, alkaline phosphatase (ALP) activity, and bony calcification assay. The results indicate that osteoblasts cultured on the composite scaffolds with different TiO₂ content (0, 0.1, and 0.3 g/1 g PLGA) display increased cell proliferation compared with pure PLGA scaffold. When cultured on composite scaffolds, osteoblasts also exhibit significantly enhanced ALP activity and higher calcium secretion, with respect to those on the pure PLGA scaffolds. Taken together, PLGA/TiO₂-SMSs deserve attention utilizing for potential bone-repairing therapeutics. © 2010 Wiley Periodicals, Inc.

Original language	English
Pages (from-to)	84-92
Journal	Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume	93
Issue number	1
DOIs	https://doi.org/10.1002/jbm.b.31561
Publication status	Published - Apr 2010
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Bone repair
Microspheres
PLGA
Scaffold
TiO2 nanoparticles

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title = "Poly(lactide-co-glycolide)/titania composite microsphere-sintered scaffolds for bone tissue engineering applications",

abstract = "The objective of this study was to synthesize and characterize novel threedimensional porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-TiO2-particle composite microspheres for potential bone repair applications. The introduction of TiO2 component has been proven capable of largely enhancing mechanical properties of PLGA/ TiO 2 microsphere-sintered scaffold ({"}PLGA/TiO2- SMS{"}). In addition, composite nano-TiO2 additives are capable of inducing an increased arrest of adhesive proteins from the environment, which benefits cell attachment onto the scaffolds. Osteoblast proliferation and maturation were evaluated by MTT assay, alkaline phosphatase (ALP) activity, and bony calcification assay. The results indicate that osteoblasts cultured on the composite scaffolds with different TiO2 content (0, 0.1, and 0.3 g/1 g PLGA) display increased cell proliferation compared with pure PLGA scaffold. When cultured on composite scaffolds, osteoblasts also exhibit significantly enhanced ALP activity and higher calcium secretion, with respect to those on the pure PLGA scaffolds. Taken together, PLGA/TiO2-SMSs deserve attention utilizing for potential bone-repairing therapeutics. {\textcopyright} 2010 Wiley Periodicals, Inc.",

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Poly(lactide-co-glycolide)/titania composite microsphere-sintered scaffolds for bone tissue engineering applications. / Wang, Yingjun; Shi, Xuetao; Ren, Li et al.
In: Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 93, No. 1, 04.2010, p. 84-92.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Poly(lactide-co-glycolide)/titania composite microsphere-sintered scaffolds for bone tissue engineering applications

AU - Wang, Yingjun

AU - Shi, Xuetao

AU - Ren, Li

AU - Yao, Yongchang

AU - Zhang, Feng

AU - Wang, Dong-An

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

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N2 - The objective of this study was to synthesize and characterize novel threedimensional porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-TiO2-particle composite microspheres for potential bone repair applications. The introduction of TiO2 component has been proven capable of largely enhancing mechanical properties of PLGA/ TiO 2 microsphere-sintered scaffold ("PLGA/TiO2- SMS"). In addition, composite nano-TiO2 additives are capable of inducing an increased arrest of adhesive proteins from the environment, which benefits cell attachment onto the scaffolds. Osteoblast proliferation and maturation were evaluated by MTT assay, alkaline phosphatase (ALP) activity, and bony calcification assay. The results indicate that osteoblasts cultured on the composite scaffolds with different TiO2 content (0, 0.1, and 0.3 g/1 g PLGA) display increased cell proliferation compared with pure PLGA scaffold. When cultured on composite scaffolds, osteoblasts also exhibit significantly enhanced ALP activity and higher calcium secretion, with respect to those on the pure PLGA scaffolds. Taken together, PLGA/TiO2-SMSs deserve attention utilizing for potential bone-repairing therapeutics. © 2010 Wiley Periodicals, Inc.

AB - The objective of this study was to synthesize and characterize novel threedimensional porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-TiO2-particle composite microspheres for potential bone repair applications. The introduction of TiO2 component has been proven capable of largely enhancing mechanical properties of PLGA/ TiO 2 microsphere-sintered scaffold ("PLGA/TiO2- SMS"). In addition, composite nano-TiO2 additives are capable of inducing an increased arrest of adhesive proteins from the environment, which benefits cell attachment onto the scaffolds. Osteoblast proliferation and maturation were evaluated by MTT assay, alkaline phosphatase (ALP) activity, and bony calcification assay. The results indicate that osteoblasts cultured on the composite scaffolds with different TiO2 content (0, 0.1, and 0.3 g/1 g PLGA) display increased cell proliferation compared with pure PLGA scaffold. When cultured on composite scaffolds, osteoblasts also exhibit significantly enhanced ALP activity and higher calcium secretion, with respect to those on the pure PLGA scaffolds. Taken together, PLGA/TiO2-SMSs deserve attention utilizing for potential bone-repairing therapeutics. © 2010 Wiley Periodicals, Inc.

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KW - PLGA

KW - Scaffold

KW - TiO2 nanoparticles

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JO - Journal of Biomedical Materials Research Part B: Applied Biomaterials

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IS - 1

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Poly(lactide-co-glycolide)/titania composite microsphere-sintered scaffolds for bone tissue engineering applications

Abstract

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