Polarity-Sensitive Probe for Two-Photon Fluorescence Lifetime Imaging of Lipid Droplets In Vitro and In Vivo

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

6 Scopus Citations
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Author(s)

  • Jie Sha
  • Weimin Liu
  • Xiuli Zheng
  • Yimin Guo
  • Xuewei Li
  • Haohui Ren
  • Yuanyuan Qin
  • Jiasheng Wu
  • Pengfei Wang

Detail(s)

Original languageEnglish
Pages (from-to)15350-15356
Journal / PublicationAnalytical Chemistry
Volume95
Issue number41
Online published2 Oct 2023
Publication statusPublished - 17 Oct 2023

Abstract

Lipid droplets (LDs) are crucial organelles used to store lipids and participate in lipid metabolism in cells. The abnormal aggregation and polarity change of LDs are associated with the occurrence of diseases, such as steatosis. Herein, the polarity-sensitive probe TBPCPP with a donor−acceptor-π-acceptor (D-A-π-A) structure was designed and synthesized. The TBPCPP has a large Stokes shift (∼220 nm), excellent photostability, high LD targeting, and considerable two-photon absorption (TPA) cross-section (∼226 GM), enabling deep two-photon imaging (∼360 μm). In addition, the fluorescence lifetime of TBPCPP decreases linearly with increasing solvent polarity. Therefore, with the assistance of two-photon fluorescence lifetime imaging microscopy (TP-FLIM), TBPCPP has successfully achieved not only the visualization of polarity changes caused by LD accumulation in HepG-2 cells but also lipid-specific imaging and visualization of different polarities in lipid-rich regions in zebrafish for the first time. Furthermore, TP-FLIM revealed that the polarity gradually decreases during steatosis in HepG-2 cells, which provided new insights into the diagnosis of steatosis. © 2023 American Chemical Society.

Citation Format(s)

Polarity-Sensitive Probe for Two-Photon Fluorescence Lifetime Imaging of Lipid Droplets In Vitro and In Vivo. / Sha, Jie; Liu, Weimin; Zheng, Xiuli et al.
In: Analytical Chemistry, Vol. 95, No. 41, 17.10.2023, p. 15350-15356.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review