TY - GEN
T1 - Plasmonic metallic nanoparticle-based biosensors
AU - Lee, Jung-hoon
AU - Mi, Hua
N1 - Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).
PY - 2023
Y1 - 2023
N2 - Bifunctional nanoparticle of combining magnetic and plasmonic nanomaterials retain both unique properties, contributing to the high photothermal performance, excellent biocompatibility, physiological stability, low cytotoxicity and easy separation. Herein, we report a core-shell plasmonic magnetic nanostructure (PMNs), then introduce the plasmonic photothermal polymerase chain reaction (PPT-PCR) platform for fast, sensitive, cheap, and simple nucleic acid detection based on PMNs. Magnetic nanoparticles can be synthesized by solvothermal reaction. PMNs can be prepared after Au coating on the magnetic core, which can act as nanoheater and heat solution to 95 ℃ in several seconds upon infra-red (IR) light irradiation, and can be collected by magnet easily. Furthermore, our platforms utilize ultrafast PCR amplification based on the photothermal effect of plasmonic magnetic nanoparticles for molecular diagnostics through two modes, including in-situ end-point quantitative fluorescence detection (PPT-qPCR) and colorimetric assay (PPT-cPCR), having comparable limit of detection (LOD) on DNA targets. © 2023 SPIE.
AB - Bifunctional nanoparticle of combining magnetic and plasmonic nanomaterials retain both unique properties, contributing to the high photothermal performance, excellent biocompatibility, physiological stability, low cytotoxicity and easy separation. Herein, we report a core-shell plasmonic magnetic nanostructure (PMNs), then introduce the plasmonic photothermal polymerase chain reaction (PPT-PCR) platform for fast, sensitive, cheap, and simple nucleic acid detection based on PMNs. Magnetic nanoparticles can be synthesized by solvothermal reaction. PMNs can be prepared after Au coating on the magnetic core, which can act as nanoheater and heat solution to 95 ℃ in several seconds upon infra-red (IR) light irradiation, and can be collected by magnet easily. Furthermore, our platforms utilize ultrafast PCR amplification based on the photothermal effect of plasmonic magnetic nanoparticles for molecular diagnostics through two modes, including in-situ end-point quantitative fluorescence detection (PPT-qPCR) and colorimetric assay (PPT-cPCR), having comparable limit of detection (LOD) on DNA targets. © 2023 SPIE.
KW - bifunctional nanoparticle
KW - biosensor
KW - colorimetric
KW - photothermal effect
KW - Plasmonic magnetic nanoparticle
KW - plasmonic PCR
KW - polymerase chain reaction
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U2 - 10.1117/12.2676310
DO - 10.1117/12.2676310
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781510665224
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Enhanced Spectroscopies and Nanoimaging 2023
A2 - Verma, Prabhat
A2 - Suh, Yung Doug
PB - SPIE
T2 - Enhanced Spectroscopies and Nanoimaging 2023
Y2 - 20 August 2023 through 23 August 2023
ER -