TY - JOUR
T1 - Plasmonic Cross-Linking Colorimetric PCR for Simple and Sensitive Nucleic Acid Detection
AU - Jiang, Kunlun
AU - Wu, Jingrui
AU - Kim, Ji-Eun
AU - An, Sujin
AU - Nam, Jwa-Min
AU - Peng, Yung-Kang
AU - Lee, Jung-Hoon
PY - 2023/5/10
Y1 - 2023/5/10
N2 - Simple, low-cost, and accurate nucleic acid assay platforms hold great promise for point-of-care (POC) pathogen detection, disease surveillance, and control. Plasmonic photothermal polymerase chain reaction (PPT-PCR) is a powerful and efficient nucleic acid amplification technique, but it lacks a simple and convenient analysis method for POC applications. Herein, we propose a novel plasmonic cross-linking colorimetric PCR (PPT-ccPCR) assay by integrating plasmonic magnetic nanoparticle (PMN)-based PPT-PCR with gold nanoparticle (AuNP)-based cross-linking colorimetry. AuNPs form assembled structures with the PMNs in the presence of amplicons and collect in a magnetic field, resulting in color changes to the supernatant. Target DNA with concentrations as low as 5 copies/μL can be visually detected within 40 min. The achieved limit of detection was 1.8 copies/μL based on the absorption signals. This simple and sensitive strategy needs no expensive instrumentation and demonstrates high potential for POC detection while enabling further applications in clinical diagnostics. © 2023 American Chemical Society.
AB - Simple, low-cost, and accurate nucleic acid assay platforms hold great promise for point-of-care (POC) pathogen detection, disease surveillance, and control. Plasmonic photothermal polymerase chain reaction (PPT-PCR) is a powerful and efficient nucleic acid amplification technique, but it lacks a simple and convenient analysis method for POC applications. Herein, we propose a novel plasmonic cross-linking colorimetric PCR (PPT-ccPCR) assay by integrating plasmonic magnetic nanoparticle (PMN)-based PPT-PCR with gold nanoparticle (AuNP)-based cross-linking colorimetry. AuNPs form assembled structures with the PMNs in the presence of amplicons and collect in a magnetic field, resulting in color changes to the supernatant. Target DNA with concentrations as low as 5 copies/μL can be visually detected within 40 min. The achieved limit of detection was 1.8 copies/μL based on the absorption signals. This simple and sensitive strategy needs no expensive instrumentation and demonstrates high potential for POC detection while enabling further applications in clinical diagnostics. © 2023 American Chemical Society.
KW - Biosensor
KW - Colorimetric
KW - Nucleic acid detection
KW - Photothermal effect
KW - Plasmonic photothermal PCR
UR - http://www.scopus.com/inward/record.url?scp=85154053249&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85154053249&origin=recordpage
U2 - 10.1021/acs.nanolett.3c00533
DO - 10.1021/acs.nanolett.3c00533
M3 - RGC 21 - Publication in refereed journal
SN - 1530-6984
VL - 23
SP - 3897
EP - 3903
JO - Nano Letters
JF - Nano Letters
IS - 9
ER -