Plasmonic Cross-Linking Colorimetric PCR for Simple and Sensitive Nucleic Acid Detection

Kunlun Jiang, Jingrui Wu, Ji-Eun Kim, Sujin An, Jwa-Min Nam, Yung-Kang Peng*, Jung-Hoon Lee*

*Corresponding author for this work

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

17 Citations (Scopus)

Abstract

Simple, low-cost, and accurate nucleic acid assay platforms hold great promise for point-of-care (POC) pathogen detection, disease surveillance, and control. Plasmonic photothermal polymerase chain reaction (PPT-PCR) is a powerful and efficient nucleic acid amplification technique, but it lacks a simple and convenient analysis method for POC applications. Herein, we propose a novel plasmonic cross-linking colorimetric PCR (PPT-ccPCR) assay by integrating plasmonic magnetic nanoparticle (PMN)-based PPT-PCR with gold nanoparticle (AuNP)-based cross-linking colorimetry. AuNPs form assembled structures with the PMNs in the presence of amplicons and collect in a magnetic field, resulting in color changes to the supernatant. Target DNA with concentrations as low as 5 copies/μL can be visually detected within 40 min. The achieved limit of detection was 1.8 copies/μL based on the absorption signals. This simple and sensitive strategy needs no expensive instrumentation and demonstrates high potential for POC detection while enabling further applications in clinical diagnostics. © 2023 American Chemical Society.
Original languageEnglish
Pages (from-to)3897–3903
JournalNano Letters
Volume23
Issue number9
Online published21 Apr 2023
DOIs
Publication statusPublished - 10 May 2023

Research Keywords

  • Biosensor
  • Colorimetric
  • Nucleic acid detection
  • Photothermal effect
  • Plasmonic photothermal PCR

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