Plasma-treated polyethylene surfaces for improved binding of active protein

Joan P.Y. Ho; Neil J. Nosworthy; Marcela M.M. Bilek; Bee K. Gan; David R. McKenzie; Paul K. Chu; Cris G. dos Remedios

doi:10.1002/ppap.200600182

Plasma-treated polyethylene surfaces for improved binding of active protein

Joan P.Y. Ho, Neil J. Nosworthy, Marcela M.M. Bilek, Bee K. Gan, David R. McKenzie, Paul K. Chu, Cris G. dos Remedios

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

43 Citations (Scopus)

Abstract

The use of plasma immersion ion implantation (PIII) to prepare polyethylene surfaces for binding active proteins is investigated. The PIII surface treatment significantly improves the density of active HRP bound to the surface after incubation in buffer containing the protein. The retention of enzyme activity on the PIII-treated surfaces is greatly improved as compared to both untreated controls and surfaces exposed to the plasma without the PIII treatment. A nitrogen PIII treatment produced surfaces with the best retention of protein activity. Oxidation of the ion-damaged surface and the formation of a carbonized subsurface are believed to be responsible for the observed protein binding properties. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	583-590
Journal	Plasma Processes and Polymers
Volume	4
Issue number	5
DOIs	https://doi.org/10.1002/ppap.200600182
Publication status	Published - 25 Jul 2007

Research Keywords

Enzyme activity
Horseradish peroxidase
Plasma immersion ion implantation
Polyethylene
Proteins

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title = "Plasma-treated polyethylene surfaces for improved binding of active protein",

abstract = "The use of plasma immersion ion implantation (PIII) to prepare polyethylene surfaces for binding active proteins is investigated. The PIII surface treatment significantly improves the density of active HRP bound to the surface after incubation in buffer containing the protein. The retention of enzyme activity on the PIII-treated surfaces is greatly improved as compared to both untreated controls and surfaces exposed to the plasma without the PIII treatment. A nitrogen PIII treatment produced surfaces with the best retention of protein activity. Oxidation of the ion-damaged surface and the formation of a carbonized subsurface are believed to be responsible for the observed protein binding properties. {\textcopyright} 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",

keywords = "Enzyme activity, Horseradish peroxidase, Plasma immersion ion implantation, Polyethylene, Proteins",

author = "Ho, {Joan P.Y.} and Nosworthy, {Neil J.} and Bilek, {Marcela M.M.} and Gan, {Bee K.} and McKenzie, {David R.} and Chu, {Paul K.} and {dos Remedios}, {Cris G.}",

year = "2007",

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language = "English",

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journal = "Plasma Processes and Polymers",

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TY - JOUR

T1 - Plasma-treated polyethylene surfaces for improved binding of active protein

AU - Ho, Joan P.Y.

AU - Nosworthy, Neil J.

AU - Bilek, Marcela M.M.

AU - Gan, Bee K.

AU - McKenzie, David R.

AU - Chu, Paul K.

AU - dos Remedios, Cris G.

PY - 2007/7/25

Y1 - 2007/7/25

N2 - The use of plasma immersion ion implantation (PIII) to prepare polyethylene surfaces for binding active proteins is investigated. The PIII surface treatment significantly improves the density of active HRP bound to the surface after incubation in buffer containing the protein. The retention of enzyme activity on the PIII-treated surfaces is greatly improved as compared to both untreated controls and surfaces exposed to the plasma without the PIII treatment. A nitrogen PIII treatment produced surfaces with the best retention of protein activity. Oxidation of the ion-damaged surface and the formation of a carbonized subsurface are believed to be responsible for the observed protein binding properties. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - The use of plasma immersion ion implantation (PIII) to prepare polyethylene surfaces for binding active proteins is investigated. The PIII surface treatment significantly improves the density of active HRP bound to the surface after incubation in buffer containing the protein. The retention of enzyme activity on the PIII-treated surfaces is greatly improved as compared to both untreated controls and surfaces exposed to the plasma without the PIII treatment. A nitrogen PIII treatment produced surfaces with the best retention of protein activity. Oxidation of the ion-damaged surface and the formation of a carbonized subsurface are believed to be responsible for the observed protein binding properties. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Enzyme activity

KW - Horseradish peroxidase

KW - Plasma immersion ion implantation

KW - Polyethylene

KW - Proteins

UR - http://www.scopus.com/inward/record.url?scp=34547522811&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-34547522811&origin=recordpage

U2 - 10.1002/ppap.200600182

DO - 10.1002/ppap.200600182

M3 - RGC 21 - Publication in refereed journal

SN - 1612-8850

VL - 4

SP - 583

EP - 590

JO - Plasma Processes and Polymers

JF - Plasma Processes and Polymers

IS - 5

ER -

Plasma-treated polyethylene surfaces for improved binding of active protein

Abstract

Research Keywords

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