Picoinjection-Enabled Multitarget Loop-Mediated Isothermal Amplification for Detection of Foodborne Pathogens

Hao Yuan; Youchuang Chao; ShanShan Li; Matthew Y. H. Tang; Yue Huang; You Che; Alice S. T. Wong; Tong Zhang; Ho Cheung Shum

doi:10.1021/acs.analchem.8b03673

Picoinjection-Enabled Multitarget Loop-Mediated Isothermal Amplification for Detection of Foodborne Pathogens

Hao Yuan
, Youchuang Chao
, ShanShan Li
, Matthew Y. H. Tang
, Yue Huang
, You Che
, Alice S. T. Wong
, Tong Zhang
, Ho Cheung Shum^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

69 Citations (Scopus)

Abstract

In this study, we develop a method to detect multiple DNAs of foodborne pathogens by encapsulating emulsion droplets for loop-mediated isothermal amplification (LAMP). In contrast to the traditional bulk-phase LAMP, which involves a labor-intensive mixing process, with our method, different primers are automatically mixed with DNA samples and LAMP buffers after picoinjection. By directly observing and analyzing the fluorescence intensity of the resultant droplets, one can detect DNA from different pathogens, with a detection limit 500 times lower than that obtained by bulk-phase LAMP. We further demonstrate the ability to quantify bacteria concentration by detecting bacterial DNA in practical samples, showing great potential in monitoring water resources and their contamination by pathogenic bacteria. © 2018 American Chemical Society.

Original language	English
Pages (from-to)	13173-13177
Journal	Analytical Chemistry
Volume	90
Issue number	22
DOIs	https://doi.org/10.1021/acs.analchem.8b03673
Publication status	Published - 20 Nov 2018
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

We would like to thank Dr. Jingmei Li for her generous help on MATLAB software programing and Dr. Qingming Ma for his constructive suggestions. We thank the technical support from Double Helix company (Guangdong, China). This work was supported by the General Research Fund (Nos. 17304514, 17306315, 17304017, and 17329516) from the Research Grants Council of Hong Kong, the General Program (No. 21476189/B060201), and the Major Research plan (No. 91434202) from the National Natural Science Foundation of China as well as the Seed Fund for Basic Research (Nos. 201711159249, 201611159205, and 201511159280), Seed Fund for Translational and Applied Research (201711160016), and Platform Technology Funding from the University of Hong Kong.

RGC Funding Information

RGC-funded

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title = "Picoinjection-Enabled Multitarget Loop-Mediated Isothermal Amplification for Detection of Foodborne Pathogens",

abstract = "In this study, we develop a method to detect multiple DNAs of foodborne pathogens by encapsulating emulsion droplets for loop-mediated isothermal amplification (LAMP). In contrast to the traditional bulk-phase LAMP, which involves a labor-intensive mixing process, with our method, different primers are automatically mixed with DNA samples and LAMP buffers after picoinjection. By directly observing and analyzing the fluorescence intensity of the resultant droplets, one can detect DNA from different pathogens, with a detection limit 500 times lower than that obtained by bulk-phase LAMP. We further demonstrate the ability to quantify bacteria concentration by detecting bacterial DNA in practical samples, showing great potential in monitoring water resources and their contamination by pathogenic bacteria. {\textcopyright} 2018 American Chemical Society.",

author = "Hao Yuan and Youchuang Chao and ShanShan Li and Tang, \{Matthew Y. H.\} and Yue Huang and You Che and Wong, \{Alice S. T.\} and Tong Zhang and Shum, \{Ho Cheung\}",

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T1 - Picoinjection-Enabled Multitarget Loop-Mediated Isothermal Amplification for Detection of Foodborne Pathogens

AU - Yuan, Hao

AU - Chao, Youchuang

AU - Li, ShanShan

AU - Tang, Matthew Y. H.

AU - Huang, Yue

AU - Che, You

AU - Wong, Alice S. T.

AU - Zhang, Tong

AU - Shum, Ho Cheung

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2018/11/20

Y1 - 2018/11/20

N2 - In this study, we develop a method to detect multiple DNAs of foodborne pathogens by encapsulating emulsion droplets for loop-mediated isothermal amplification (LAMP). In contrast to the traditional bulk-phase LAMP, which involves a labor-intensive mixing process, with our method, different primers are automatically mixed with DNA samples and LAMP buffers after picoinjection. By directly observing and analyzing the fluorescence intensity of the resultant droplets, one can detect DNA from different pathogens, with a detection limit 500 times lower than that obtained by bulk-phase LAMP. We further demonstrate the ability to quantify bacteria concentration by detecting bacterial DNA in practical samples, showing great potential in monitoring water resources and their contamination by pathogenic bacteria. © 2018 American Chemical Society.

AB - In this study, we develop a method to detect multiple DNAs of foodborne pathogens by encapsulating emulsion droplets for loop-mediated isothermal amplification (LAMP). In contrast to the traditional bulk-phase LAMP, which involves a labor-intensive mixing process, with our method, different primers are automatically mixed with DNA samples and LAMP buffers after picoinjection. By directly observing and analyzing the fluorescence intensity of the resultant droplets, one can detect DNA from different pathogens, with a detection limit 500 times lower than that obtained by bulk-phase LAMP. We further demonstrate the ability to quantify bacteria concentration by detecting bacterial DNA in practical samples, showing great potential in monitoring water resources and their contamination by pathogenic bacteria. © 2018 American Chemical Society.

UR - https://www.scopus.com/pages/publications/85055841646

UR - https://www.scopus.com/pages/publications/85055841646#tab=impact

U2 - 10.1021/acs.analchem.8b03673

DO - 10.1021/acs.analchem.8b03673

M3 - RGC 21 - Publication in refereed journal

C2 - 30354065

SN - 0003-2700

VL - 90

SP - 13173

EP - 13177

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 22

ER -

Picoinjection-Enabled Multitarget Loop-Mediated Isothermal Amplification for Detection of Foodborne Pathogens

Abstract

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