Phylogenetic microarray analysis of a microbial community performing reductive dechlorination at a TCE-contaminated site

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • F. Warnecke
  • Eoin L. Brodie
  • Tamzen W. MacBeth
  • Mark E. Conrad
  • Gary L. Andersen
  • Lisa Alvarez-Cohen

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)1044-1054
Journal / PublicationEnvironmental Science and Technology
Volume46
Issue number2
Publication statusPublished - 17 Jan 2012

Abstract

A high-density phylogenetic microarray (PhyloChip) was applied to track bacterial and archaeal populations through different phases of remediation at Ft. Lewis, WA, a trichloroethene (TCE)-contaminated groundwater site. Biostimulation with whey, and bioaugmentation with a Dehalococcoides-containing enrichment culture were strategies implemented to enhance dechlorination. As a measure of species richness, over 1300 operational taxonomic units (OTUs) were detected in DNA from groundwater samples extracted during different stages of treatment and in the bioaugmentation culture. In order to determine active members within the community, 16S rRNA from samples were analyzed by microarray and ∼600 OTUs identified. A cDNA clone library of the expressed 16S rRNA corroborated the observed diversity and activity of some of the phyla. Principle component analysis of the treatment plot samples revealed that the microbial populations were constantly changing during the course of the study. Dynamic analysis of the archaeal population showed significant increases in methanogens at the later stages of treatment that correlated with increases in methane concentrations of over 2 orders of magnitude. Overall, the PhyloChip analyses in this study have provided insights into the microbial ecology and population dynamics at the TCE-contaminated field site useful for understanding the in situ reductive dechlorination processes. © 2011 American Chemical Society.

Citation Format(s)

Phylogenetic microarray analysis of a microbial community performing reductive dechlorination at a TCE-contaminated site. / Lee, Patrick K. H.; Warnecke, F.; Brodie, Eoin L.; MacBeth, Tamzen W.; Conrad, Mark E.; Andersen, Gary L.; Alvarez-Cohen, Lisa.

In: Environmental Science and Technology, Vol. 46, No. 2, 17.01.2012, p. 1044-1054.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review