Photothermal bleaching in time-lapse photoacoustic microscopy

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

13 Scopus Citations
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Author(s)

  • Liang Gao
  • Lidai Wang
  • Chiye Li
  • Alejandro Garcia-Uribe
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Pages (from-to)543-548
Journal / PublicationJournal of Biophotonics
Volume6
Issue number6-7
Online published26 Nov 2012
Publication statusPublished - Jun 2013
Externally publishedYes

Abstract

We studied the phenomenon of photothermal bleaching - a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy, pulse duration, and the absorber's size was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time-lapse imaging applications.

Research Area(s)

  • Nanoparticle, Photoacoustic microscopy, Photothermal bleaching, Timelapse imaging

Citation Format(s)

Photothermal bleaching in time-lapse photoacoustic microscopy. / Gao, Liang; Wang, Lidai; Li, Chiye et al.
In: Journal of Biophotonics, Vol. 6, No. 6-7, 06.2013, p. 543-548.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review