Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)938-945
Journal / PublicationMarine Pollution Bulletin
Volume124
Issue number2
Online published30 Mar 2017
Publication statusPublished - 30 Nov 2017

Abstract

A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200 μg/L Cu, EC50 for the yield at 25 °C; and 1000 μg/L Cu, EC50 for growth inhibition at 25 °C) were conducted against four temperatures (10 °C, 15 °C, 25 °C and 30 °C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30 °C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90–2 and sit1 (related to silica shell formation) were highly expressed at 30 °C under 1000 μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15 °C under 200 μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.

Research Area(s)

  • Copper, Heat shock protein, Marine diatom, Photosynthesis, Thalassiosira, Thermal stress

Citation Format(s)

Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure. / Leung, Priscilla T.Y; Yi, Andy Xianliang; Ip, Jack C.H. et al.

In: Marine Pollution Bulletin, Vol. 124, No. 2, 30.11.2017, p. 938-945.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review