Photo-imprint super-resolution photoacoustic microscopy

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

2 Scopus Citations
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Author(s)

  • Junjie Yao
  • Lidai Wang
  • Chiye Li
  • Chi Zhang
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9323
ISBN (print)9781628414134
Publication statusPublished - 2015
Externally publishedYes

Publication series

Name
Volume9323
ISSN (Print)1605-7422

Conference

TitlePhotons Plus Ultrasound: Imaging and Sensing 2015
PlaceUnited States
CitySan Francisco
Period8 - 10 February 2015

Abstract

Combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for super-resolution photoacoustic imaging of both fluorescent and non-fluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. By scanning the excitation beam, we performed three-dimensional sub-diffraction imaging of varied fluorescent and non-fluorescent species. A lateral resolution of 80 nm and an axial resolution of 370 nm have been demonstrated. This technique has the potential to enable label-free super-resolution imaging, and can be transferred to other optical imaging modalities or combined with other super-resolution methods.

Research Area(s)

  • Label-free, Non-fluorescent imaging, Photoacoustic microscopy, Sub-diffraction imaging, Super-resolution imaging

Citation Format(s)

Photo-imprint super-resolution photoacoustic microscopy. / Yao, Junjie; Wang, Lidai; Li, Chiye et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9323 SPIE, 2015. 932327.

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review